Repository logo
 
Publication

Endocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and PTHrP

2014-05Journal article Open access
Simple item page
dc.contributor.authorGregorio, Silvia F.
dc.contributor.authorCarvalho, Edison S. M.
dc.contributor.authorCampinho, Marco A.
dc.contributor.authorPower, Deborah M.
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorFuentes, Juan
dc.date.accessioned2018-12-07T14:53:49Z
dc.date.available2018-12-07T14:53:49Z
dc.date.issued2014-05
dc.description.abstractIn marine fish, high epithelial bicarbonate secretion by the intestine generates luminal carbonate precipitates of divalent cations that play a key role in water and ion homeostasis. In vitro studies highlight the involvement of the calciotropic hormones PTHrP (parathyroid hormone-related protein) and stanniocalcin (STC) in the regulation of epithelial bicarbonate transport. The present study tested the hypothesis that calciotropic hormones have a regulatory role in carbonate precipitate formation in vivo. Sea bream (Sparus aurata) juveniles received single intraperitoneal injections of piscine PTHrP(1-34), the PTH/PTHrP receptor antagonist PTHrP(7-34) or purified sea bream STC, or were passively immunized with polyclonal rabbit antisera raised against sea bream STC (STC-Ab). Endocrine effects on the expression of the basolateral sodium bicarbonate cotransporter (Slc4a4.A), the apical anion exchangers Slc26a6.A and Slc26a3.B, and the V-type proton pump beta-subunit (Atp6v1b) in the anterior intestine were evaluated. In keeping with their calciotropic nature, the hypocalcaemic factors PTHrP(7-34) and STC upregulated gene expression of all transporters. In contrast, the hypercalcaemic factor PTHrP(1-34) and STC antibodies downregulated transporters involved in the bicarbonate secretion cascade. Changes in intestine luminal precipitate contents provoked by calcaemic endocrine factors validated these results: 24 h postinjection either PTHrP(1-34) or immunization with STC-Ab reduced the carbonate precipitate content in the sea bream intestine. In contrast, the PTH/PTHrP receptor antagonist PTHrP(7-34) increased not only the precipitated fraction but also the concentration of HCO3 equivalents in the intestinal fluid. These results confirm the hypothesis that calciotropic hormones have a regulatory role in carbonate precipitate formation in vivo in the intestine of marine fish. Furthermore, they illustrate for the first time in fish the counteracting effect of PTHrP and STC, and reveal an unexpected contribution of calcaemic factors to acid-base balance.
dc.description.sponsorshipPortuguese Foundation for Science and Technology (Ministry of Science and Higher Education, Portugal and European Social Funds) [PTDC/MAR/104008/2008, PTDC/MAR-BIO/3811/2012, SFRH/BPD/66808/2009]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1242/jeb.098517
dc.identifier.issn0022-0949
dc.identifier.issn1477-9145
dc.identifier.urihttp://hdl.handle.net/10400.1/11706
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCompany Of Biologists Ltd
dc.relationMOLECULAR AND CELLULAR EFFECTS OF IOXINIL AND DIETHYLSTILBESTROL ON ZEBRAFISH THYROCYTE DEVELOPMENT AND ADULT HOMEOSTASIS
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHormone-related protein
dc.subjectSparus-Aurata L.
dc.subjectSoluble Adenylyl-Cyclase
dc.subjectSalmo-Salar L
dc.subjectBicarbonate secretion
dc.subjectSea bream
dc.subjectParathyroid-hormone
dc.subjectHco3-secretion
dc.subjectTeleost Fish
dc.subjectWater-absorption
dc.titleEndocrine regulation of carbonate precipitate formation in marine fish intestine by stanniocalcin and PTHrP
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMOLECULAR AND CELLULAR EFFECTS OF IOXINIL AND DIETHYLSTILBESTROL ON ZEBRAFISH THYROCYTE DEVELOPMENT AND ADULT HOMEOSTASIS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F104008%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR-BIO%2F3811%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F66808%2F2009/PT
oaire.citation.endPage1562
oaire.citation.issue9
oaire.citation.startPage1555
oaire.citation.titleJournal of Experimental Biology
oaire.citation.volume217
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNameGregorio
person.familyNameCarvalho
person.familyNameCampinho
person.familyNamePower
person.familyNameCanario
person.familyNameFuentes
person.givenNameSilvia
person.givenNameEdison SM
person.givenNameMarco António
person.givenNameDeborah Mary
person.givenNameAdelino
person.givenNameJuan
person.identifier1579837
person.identifier143624
person.identifier392998
person.identifier.ciencia-idBF14-020B-4C39
person.identifier.ciencia-idCD11-5971-044A
person.identifier.ciencia-id0E18-2560-6EC1
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id421B-E196-2C33
person.identifier.orcid0000-0002-6648-5122
person.identifier.orcid0000-0002-4970-9278
person.identifier.orcid0000-0002-5238-0506
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-0430-8734
person.identifier.ridG-1618-2018
person.identifier.ridD-8833-2013
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id8938999600
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7201832526
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication9bc940d8-7dfb-4950-98f4-6853f398cb49
relation.isAuthorOfPublicationd3dea3a5-6406-436d-87ae-486e5b7b005f
relation.isAuthorOfPublicationf8e8c466-6a6f-4ccf-b96d-34565fe53b6c
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublicationd354b937-b18c-4ca5-a843-04a04269d60f
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isProjectOfPublicationf2957511-15a2-40dc-9778-6eb01edd3508
relation.isProjectOfPublication3617ac94-04d3-44a9-b636-f9566b0860d0
relation.isProjectOfPublicationa6a1194c-067d-4a72-abc6-7387f936b274
relation.isProjectOfPublication.latestForDiscoveryf2957511-15a2-40dc-9778-6eb01edd3508

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
handle11706.pdf
Size:
643.87 KB
Format:
Adobe Portable Document Format
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)