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Characterization of plasma SDS-protein aggregation profile of patients with heart failure with preserved ejection fraction

2022Journal article Restricted
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dc.contributor.authorGouveia, Marisol
dc.contributor.authorSchmidt, Cristine
dc.contributor.authorTeixeira, Manuel
dc.contributor.authorLopes, Mário
dc.contributor.authorAveiro, Susana
dc.contributor.authorDomingues, Pedro
dc.contributor.authorXia, Ke
dc.contributor.authorColón, Wilfredo
dc.contributor.authorVitorino, Rui
dc.contributor.authorFerreira, Rita
dc.contributor.authorSantos, Mário
dc.contributor.authorVieira, Sandra
dc.contributor.authorRibeiro, Fernando
dc.date.accessioned2023-01-23T15:26:12Z
dc.date.available2023-01-23T15:26:12Z
dc.date.issued2022
dc.description.abstractThis study characterizes the plasma levels and composition of SDS-resistant aggregates (SRAs) in patients with heart failure with preserved ejection fraction (HFpEF) to infer molecular pathways associated with disease and/or proteostasis disruption. Twenty adults (ten with HFpEF and ten age-matched individuals) were included. Circulating SRAs were resolved by diagonal two-dimensional SDS-PAGE, and their protein content was identified by mass spectrometry. Protein carbonylation, ubiquitination and ficolin-3 were evaluated. Patients with HFpEF showed higher SRA/total (36.6 +/- 4.9% vs 29.6 +/- 2.2%, p = 0.009) and SRA/soluble levels (58.6 +/- 12.7% vs 40.6 +/- 5.8%, p = 0.008). SRAs were carbonylated and ubiquitinated, suggesting they are composed of dysfunctional proteins resistant to degradation. SRAs were enriched in proteins associated with cardiovascular function/disease and with proteostasis machinery. Total ficolin-3 levels were decreased (0.77 +/- 0.22, p = 0.041) in HFpEF, suggesting a reduced proteostasis capacity to clear circulating SRA. Thus, the higher accumulation of SRA in HFpEF may result from a failure or overload of the protein clearance machinery.pt_PT
dc.description.sponsorshipBEX0554/14-6
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s12265-022-10334-wpt_PT
dc.identifier.eissn1937-5395
dc.identifier.urihttp://hdl.handle.net/10400.1/18896
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationUnit for Multidisciplinary Research in Biomedicine
dc.relationUnit for Multidisciplinary Research in Biomedicine
dc.relationAnalysis of protein aggregation in plasma of patients with heart failure with preserved ejection fraction to find novel therapeutic targets and monitor therapeutic interventions
dc.relationTargeting pulmonary artery endothelial cells dysfunction in pulmonary arterial hypertension: in situ assessment of the effects of acute exercise
dc.subjectCardiovascular diseasept_PT
dc.subjectProteostasispt_PT
dc.subjectAggregatespt_PT
dc.subjectMass spectrometrypt_PT
dc.subjectBioinformaticspt_PT
dc.titleCharacterization of plasma SDS-protein aggregation profile of patients with heart failure with preserved ejection fractionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleUnit for Multidisciplinary Research in Biomedicine
oaire.awardTitleUnit for Multidisciplinary Research in Biomedicine
oaire.awardTitleAnalysis of protein aggregation in plasma of patients with heart failure with preserved ejection fraction to find novel therapeutic targets and monitor therapeutic interventions
oaire.awardTitleTargeting pulmonary artery endothelial cells dysfunction in pulmonary arterial hypertension: in situ assessment of the effects of acute exercise
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00215%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00215%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F128893%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/2020.08565.BD/PT
oaire.citation.titleJournal of Cardiovascular Translational Researchpt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_CENTRO
oaire.fundingStreamPOR_CENTRO
person.familyNameAveiro
person.givenNameSusana
person.identifier.ciencia-idD312-07B4-DA74
person.identifier.orcid0000-0002-6651-3226
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoveryed8b83e5-9fb0-4701-a65e-90690ccc139c
relation.isProjectOfPublication23f88acf-6c29-4c4d-9321-9d0a491e644f
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