Publication
Direct PCR detection of foot-and-mouth disease virus
| dc.contributor.author | Rodriguez, A. | |
| dc.contributor.author | Nunez, J. | |
| dc.contributor.author | Nolasco, Gustavo | |
| dc.contributor.author | Ponz, F. | |
| dc.contributor.author | Sobrino, F. | |
| dc.contributor.author | Deblas, C. | |
| dc.date.accessioned | 2015-06-15T14:13:45Z | |
| dc.date.available | 2015-06-15T14:13:45Z | |
| dc.date.issued | 1994 | |
| dc.description.abstract | A PCR assay for the detection and characterization of foot-and-mouth disease virus was developed. The procedure allows RT-PCR amplification following direct adsorption of viral suspensions to microtiter plates, avoiding previous steps of phenol-extraction or heating. Using this procedure, FMDV-specific (based on 3D gene sequences), as well as serotype-specific (based on VP1 gene sequences) amplification were achieved for viral samples of serotypes A, O and C, either from cell culture supernatants or from lesions of infected animals. The assay allowed detection of around 15 PFU, being 500-fold more sensitive than a conventional indirect ELISA. This new method constitutes a simple, rapid and efficient alternative for the diagnosis and characterization of FMDV by PCR. | |
| dc.identifier.doi | https://dx.doi.org/10.1016/0166-0934(94)90030-2 | |
| dc.identifier.issn | 0166-0934 | |
| dc.identifier.other | AUT: GNO00324; | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/6228 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Elsevier | |
| dc.relation.isbasedon | P-008-VWC | |
| dc.title | Direct PCR detection of foot-and-mouth disease virus | |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 349 | |
| oaire.citation.startPage | 345 | |
| oaire.citation.title | Journal of Virological Methods | |
| oaire.citation.volume | 47 | |
| person.familyName | Nolasco | |
| person.givenName | Gustavo | |
| person.identifier.orcid | 0000-0002-7025-6023 | |
| person.identifier.scopus-author-id | 6602625528 | |
| rcaap.rights | restrictedAccess | |
| rcaap.type | article | |
| relation.isAuthorOfPublication | 87a8035f-bc15-44dd-b8c3-4288c5660fff | |
| relation.isAuthorOfPublication.latestForDiscovery | 87a8035f-bc15-44dd-b8c3-4288c5660fff |