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Cartilage acidic protein 1, a new member of the beta-propeller protein family with amyloid propensity

2017-02Journal article Open Access
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dc.contributor.authorAnjos, Lliana
dc.contributor.authorMorgado, Isabel
dc.contributor.authorGuerreiro, Marta
dc.contributor.authorCardoso, João CR
dc.contributor.authorMelo, Eduardo
dc.contributor.authorPower, Deborah
dc.date.accessioned2019-11-20T15:07:50Z
dc.date.available2019-11-20T15:07:50Z
dc.date.issued2017-02
dc.description.abstractCartilage acidic protein1 (CRTAC1) is an extracellular matrix protein of chondrogenic tissue in humans and its presence in bacteria indicate it is of ancient origin. Structural modeling of piscine CRTAC1 reveals it belongs to the large family of beta-propeller proteins that in mammals have been associated with diseases, including amyloid diseases such as Alzheimer's. In order to characterize the structure/function evolution of this new member of the beta-propeller family we exploited the unique characteristics of piscine duplicate genes Crtac1a and Crtac1b and compared their structural and biochemical modifications with human recombinant CRTAC1. We demonstrate that CRTAC1 has a beta-propeller structure that has been conserved during evolution and easily forms high molecular weight thermo-stable aggregates. We reveal for the first time the propensity of CRTAC1 to form amyloid-like structures, and hypothesize that the aggregating property of CRTAC1 may be related to its disease-association. We further contribute to the general understating of CRTAC1's and beta-propeller family evolution and function. Proteins 2017; 85:242-255. (c) 2016 Wiley Periodicals, Inc.
dc.description.sponsorshipEuropean Regional Development Fund through COMPETE
dc.description.sponsorshipFCT project [CMAR/Multi/04326/2013]
dc.description.sponsorshipEuropean Commission MSC International Outgoing Fellowship (IOF) [628077]
dc.description.sponsorshipFCT
dc.description.sponsorshipMinistry of Science and Higher Education [SFRH/BPD/79105/2011]
dc.identifier.doi10.1002/prot.25210
dc.identifier.issn0887-3585
dc.identifier.issn1097-0134
dc.identifier.urihttp://hdl.handle.net/10400.1/13240
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relationBioprospecting function of an ancient gene (CRTAC) encoding a novel protein in a marine teleost by comparative analysis
dc.subjectEpidermal-growth-factor
dc.subjectMultiple-sclerosis
dc.subjectConnectivity prediction
dc.subjectOlfactomedin domain
dc.subjectCrystal-structure
dc.subjectGene-expression
dc.subjectSequence
dc.subjectAggregation
dc.subjectBiomarkers
dc.subjectMyocilin
dc.titleCartilage acidic protein 1, a new member of the beta-propeller protein family with amyloid propensity
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBioprospecting function of an ancient gene (CRTAC) encoding a novel protein in a marine teleost by comparative analysis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F122296%2F2010/PT
oaire.citation.endPage255
oaire.citation.issue2
oaire.citation.startPage242
oaire.citation.titleProteins-Structure Function and Bioinformatics
oaire.citation.volume85
oaire.fundingStream3599-PPCDT
person.familyNameAnjos Guerreiro
person.familyNameMorgado
person.familyNameCardoso
person.familyNamePinho Melo
person.familyNamePower
person.givenNameLiliana Isabel Tomé
person.givenNameIsabel
person.givenNameJoão
person.givenNameEduardo
person.givenNameDeborah Mary
person.identifierAAK-3800-2020
person.identifier14332
person.identifier1443188
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person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-9474-522X
person.identifier.orcid0000-0002-7826-997X
person.identifier.orcid0000-0001-7890-0170
person.identifier.orcid0000-0002-0974-8977
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridJ-4798-2013
person.identifier.ridM-4151-2013
person.identifier.scopus-author-id8575757700
person.identifier.scopus-author-id18134268300
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id35566177900
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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