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Bacterial 16S ribosomal gene fingerprints as a tool to diagnose and mitigate fish larvae gut dysbiosis

2025-10Journal article Open access
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dc.contributor.authorNAJAFPOUR, BABAK
dc.contributor.authorCanario, Adelino
dc.contributor.authorPower, Deborah Mary
dc.date.accessioned2025-10-23T09:20:39Z
dc.date.available2025-10-23T09:20:39Z
dc.date.issued2025-10
dc.description.abstractDysbiosis is associated with shifts in the diversity or relative abundance of beneficial versus harmful bacteria, leading to health issues in organisms. This study investigated gut bacterial dysbiosis associated with larval quality using 16S rRNA gene sequencing. The gut microbiome of gilthead sea bream and European sea bass, key commercial species and vertebrate models, was examined in high- and low-quality larvae batches from several European hatcheries. Larval quality, hatchery site and species influenced bacterial diversity in the gut. Individuals from larval batches that performed well had higher microbial diversity in the gut and individuals from batches that performed poorly had a gut microbiota dominated by pathogenic Vibrio (e.g., V. aestuarianus and V. cortegadensis). The bacterial dysbiosis index revealed a notable predominance of Fusobacteriota and Firmicutes phyla, Thermoanaerobacteria class and Lactobacillaceae, Moritellaceae, Clostridiaceae, Thiotrichaceae and Shewanellaceae families in good-quality larvae batches, and a prevalence of the Proteobacteria phylum, Gammaproteobacteria class, Sphingomonadaceae and Vibrionaceae families in the gut of individuals from poor-quality larvae batches. A positive dysbiosis index (cutoff >0.4) was associated with a high risk of decreased larval performance and quality. Additionally, the abundance of Clostridium_sensu_ stricto_15, Shewanellaceae_unclassified, Cetobacterium, Psychrilyobacter, Moritella and Latilactobacillus genera in the gut of good production batches, and the Vibrio genus in the gut of poor production batches, identified these genus as potential markers for diagnosing and mitigating bacterial dysbiosis in fish and potentially other vertebrates.eng
dc.description.sponsorshipprojects EMBRC.PT ALG-01-0145-FEDER-022121 and BIODATA.PT ALG-01-0145-FEDER-022231
dc.identifier.doi10.1111/1758-2229.70187
dc.identifier.eissn1758-2229
dc.identifier.issn1758-2229
dc.identifier.urihttp://hdl.handle.net/10400.1/27847
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relationCentre for Marine and Environmental Research
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relation.ispartofEnvironmental Microbiology Reports
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBacterial diversity
dc.subjectDysbiosis
dc.subjectLarval performance
dc.subjectMediterranean hatcheries
dc.subjectPathogens
dc.titleBacterial 16S ribosomal gene fingerprints as a tool to diagnose and mitigate fish larvae gut dysbiosiseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.citation.issue5
oaire.citation.titleEnvironmental Microbiology Reports
oaire.citation.volume17
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameNAJAFPOUR
person.familyNameCanario
person.familyNamePower
person.givenNameBABAK
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifier143624
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-2977-482X
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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