dc.contributor.author	Campos, C.
dc.contributor.author	Sundaram, Arvind Y. M.
dc.contributor.author	Valente, L. M. P.
dc.contributor.author	Conceição, L. E. C.
dc.contributor.author	Engrola, S.
dc.contributor.author	Fernandes, Jorge M. O.
dc.date.accessioned	2014-11-14T11:43:08Z
dc.date.available	2014-11-14T11:43:08Z
dc.date.issued	2014-06-25
dc.date.updated	2014-09-30T17:06:48Z
dc.description.abstract	Several miRNAs are known to control myogenesis in vertebrates. Some of them are specifically expressed in muscle while others have a broader tissue expression but are still involved in establishing the muscle phenotype. In teleosts, water temperature markedly affects embryonic development and larval growth. It has been previously shown that higher embryonic temperatures promoted faster development and increased size of Senegalese sole (Solea senegalensis) larvae relatively to a lower temperature. The role of miRNAs in thermal-plasticity of growth is hitherto unknown. Hence, we have used high-throughput SOLiD sequencing to determine potential changes in the miRNA transcriptome in Senegalese sole embryos that were incubated at 15°C or 21°C until hatching and then reared at a common temperature of 21°C. Results: We have identified 320 conserved miRNAs in Senegalese sole, of which 48 had not been previously described in teleosts. mir-17a-5p, mir-26a, mir-130c, mir-206-3p, mir-181a-5p, mir-181a-3p and mir-199a-5p expression levels were further validated by RT- qPCR. The majority of miRNAs were dynamically expressed during early development, with peaks of expression at pre-metamorphosis or metamorphosis. Also, a higher incubation temperature (21°C) was associated with expression of some miRNAs positively related with growth (e.g., miR-17a, miR-181-5p and miR-206) during segmentation and at hatching. Target prediction revealed that these miRNAs may regulate myogenesis through MAPK and mTOR pathways. Expression of miRNAs involved in lipid metabolism and energy production (e.g., miR-122) also differed between temperatures. A miRNA that can potentially target calpain (miR-181-3p), and therefore negatively regulate myogenesis, was preferentially expressed during segmentation at 15°C compared to 21°C. Conclusions: Temperature has a strong influence on expression of miRNAs during embryonic and larval development in fish. Higher expression levels of miR-17a, miR-181-5p and miR-206-3p and down-regulation of miR-181a-3p at 21°C may promote myogenesis and are in agreement with previous studies in Senegalese sole, which reported enhanced growth at higher embryonic temperatures compared to 15°C. Moreover, miRNAs involved in lipid metabolism and energy production may also contribute to increased larval growth at 21°C compared to 15°C. Taken together, our data indicate that miRNAs may play a role in temperature-induced phenotypic plasticity of growth in teleosts.	por
dc.description.version	Peer Reviewed
dc.identifier.citation	BMC Genomics. 2014 Jun 25;15(1):525	por
dc.identifier.doi	http://dx.doi.org/10.1186/1471-2164-15-525
dc.identifier.uri	http://hdl.handle.net/10400.1/5628
dc.language.iso	eng	por
dc.peerreviewed	yes	por
dc.publisher	BioMed Central	por
dc.rights.holder	Catarina Campos et al.; licensee BioMed Central Ltd.
dc.subject	Solea senegalensis	por
dc.subject	Epigenetics	por
dc.subject	miRNA	por
dc.subject	Embryonic temperature	por
dc.subject	Myogenesis	por
dc.subject	Growth	por
dc.title	Thermal plasticity of the miRNA transcriptome during Senegalese sole development	por
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	13	por
oaire.citation.issue	(1):525	por
oaire.citation.startPage	1	por
oaire.citation.title	BMC Genomics	por
oaire.citation.volume	15	por
person.familyName	Engrola
person.givenName	Sofia
person.identifier	237846
person.identifier.ciencia-id	A610-29E7-9B83
person.identifier.orcid	0000-0002-5244-5541
person.identifier.rid	A-2485-2012
person.identifier.scopus-author-id	8669620600
rcaap.rights	openAccess	por
rcaap.type	article	por
relation.isAuthorOfPublication	51be5a20-df9c-4a90-85eb-51d779642caa
relation.isAuthorOfPublication.latestForDiscovery	51be5a20-df9c-4a90-85eb-51d779642caa

Thermal plasticity of the miRNA transcriptome during Senegalese sole development

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