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Alternatively spliced transcripts of Sparus aurata insulin-like growth factor 1 are differentially expressed in adult tissues and during early development

2008-06Journal article Restricted access
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dc.contributor.authorTiago, Daniel
dc.contributor.authorLaizé, Vincent
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-12T13:59:56Z
dc.date.available2014-06-12T13:59:56Z
dc.date.issued2008-06
dc.date.updated2014-06-03T09:49:39Z
dc.description.abstractSpliced variants of insulin-like growth factor 1 (IGF-1), a small peptide with a critical role in metabolism and growth, have been identified in various vertebrate species. However, despite recent functional data in mammalian systems suggesting specific roles (e.g. in muscle formation) for their pro-peptides and/or E domains, their function remains unclear. In this study, three alternatively spliced variants of Sparus aurata proIGF-1 (1a, 1b, and 1c) were identified and their expression analyzed. In adult fish, IGF-1 gene expression was observed in various soft tissues (highest levels in liver) and calcified tissues, with IGF-1c being always the most expressed isoform. In developing larvae, each isoform presented a specific pattern of expression, characterized by different onset and extent and consistent with a possible role of IGF-1a and 1b during early post-hatching events (e.g. bone or muscle formation), while IGF-1c would be rather involved in early larvae formation but probably acts in concerted action with other isoforms at later stages. We also propose that, in adults, IGF-1a and 1b isoforms may have a local action, while isoform 1c would assume a systemic action, as its mammalian counterpart. This hypothesis was further supported by in silico analysis of isoform distribution, revealing that only IGF-1c/Ea isoform has been conserved throughout evolution and that other fish isoforms (i.e. 1a and 1b) may be associated with mechanisms of osmoregulation. We finally propose that IGF-1 variants may exhibit different modes of action (systemic or local) and may be involved in different developmental and adaptive mechanisms.por
dc.identifier.citationTiago DM; Laizé V; Cancela ML. Alternatively spliced transcripts of Sparus aurata insulin-like growth factor 1 are differentially expressed in adult tissues and during early development, General and Comparative Endocrinology, 157, 2, 107-115, 2008.por
dc.identifier.doihttp://dx.doi.org/ 10.1016/j.ygcen.2008.04.006
dc.identifier.issn0016-6480
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4285
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0016648008001603por
dc.subjectPro-insulin-like growth factor 1 (proIGF-1)por
dc.subjectAlternative splicingpor
dc.subjectE domainpor
dc.subjectGilthead seabream (Sparus aurata)por
dc.titleAlternatively spliced transcripts of Sparus aurata insulin-like growth factor 1 are differentially expressed in adult tissues and during early developmentpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage115por
oaire.citation.issue2por
oaire.citation.startPage107por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume157por
person.familyNameTiago
person.familyNameLaizé
person.familyNameCancela
person.givenNameDaniel
person.givenNameVincent
person.givenNameM. Leonor
person.identifier.orcid0000-0001-8418-6292
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id14422711000
person.identifier.scopus-author-id6602982778
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication10cda3a0-7b9c-4d16-bd2a-a06df1d56d94
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscovery10cda3a0-7b9c-4d16-bd2a-a06df1d56d94

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