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Effect of antiprotozoal drugs on the proliferation of the bivalve parasite Perkinsus olseni

2005Journal article Restricted access
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dc.contributor.authorElandalloussi, L. M.
dc.contributor.authorLeite, Ricardo
dc.contributor.authorRodrigues, Pedro
dc.contributor.authorAfonso, R.
dc.contributor.authorNunes, Patrícia A.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2015-11-30T15:17:51Z
dc.date.available2015-11-30T15:17:51Z
dc.date.issued2005
dc.description.abstractThe protozoan parasite Perkinsus olseni causes severe losses among Ruditapes decussatus clams. The development of an in vitro culture of this parasite together with the use of a proliferation assay has provided the opportunity to screen for drug sensitivity of this parasite. Xenobiotics known for their antimalarial and antiprotozoal properties were tested against P. olseni. Only four of these drugs, namely cycloheximide, pyrimethamine, deferoxamine (DFO) and 2,2-bipyridyl (BIP), showed in vitro inhibitory effect on the parasite proliferation. Two in vivo experiments were designed to determine the effect of iron chelators on reducing P. olseni infection in clams. For this purpose, naturally infected clams from the Ria Formosa, Portugal, were exposed to DFO and BIP at various concentrations. In the first experiment, hemolymph samples were taken from each clam before and after treatment to determine the infection intensity and in the second experiment, clams were randomly distributed in groups of five and the parasite burden in treated and untreated groups was determined at the end of the experiment on the whole clam wet tissues. Only DFO was found to be effective in reducing in vivo P. olseni infections. In addition, acute toxicity of DFO and BIP has been determined and no mortality of Perkinsus-free clams was observed. (C) 2004 Elsevier B.V. All rights reserved.
dc.identifier.doihttps://dx.doi.org/10.1016/j.aquaculture.2004.09.038
dc.identifier.issn0044-8486
dc.identifier.otherAUT: PCA01721; LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/7218
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relation.isbasedonP-000-525
dc.titleEffect of antiprotozoal drugs on the proliferation of the bivalve parasite Perkinsus olseni
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage17
oaire.citation.startPage9
oaire.citation.titleAquaculture
oaire.citation.volume243
person.familyNameLeite
person.familyNameRodrigues
person.familyNameAfonso
person.familyNameNunes Cabrita
person.familyNameCancela
person.givenNameRicardo
person.givenNamePedro
person.givenNameRicardo
person.givenNamePatrícia
person.givenNameM. Leonor
person.identifier447622
person.identifier.ciencia-idF115-256E-C84D
person.identifier.orcid0000-0002-9622-3895
person.identifier.orcid0000-0002-9668-1204
person.identifier.orcid0000-0002-3884-9312
person.identifier.orcid0000-0002-7148-8950
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridB-3389-2008
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id8362650900
person.identifier.scopus-author-id55107531000
person.identifier.scopus-author-id7006823084
rcaap.rightsrestrictedAccess
rcaap.typearticle
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