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Human-derived NLS enhance the gene transfer efficiency of chitosan

2021Journal article Open access
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dc.contributor.authorBitoque, Diogo
dc.contributor.authorMorais, Joana
dc.contributor.authorOliveira, Ana
dc.contributor.authorSequeira, Raquel L.
dc.contributor.authorCalado, Sofia
dc.contributor.authorFortunato, Tiago M.
dc.contributor.authorSimão, Sónia
dc.contributor.authorRosa Da Costa, Ana
dc.contributor.authorSilva, Gabriela A.
dc.date.accessioned2021-04-15T17:37:06Z
dc.date.available2021-04-15T17:37:06Z
dc.date.issued2021
dc.description.abstractNuclear import is considered as one of the major limitations for non-viral gene delivery systems and the incorporation of nuclear localization signals (NLS) that mediate nuclear intake can be used as a strategy to enhance internalization of exogenous DNA. In this work, human-derived endogenous NLS peptides based on insulin growth factor binding proteins (IGFBP), namely IGFBP-3 and IGFBP-5, were tested for their ability to improve nuclear translocation of genetic material by non-viral vectors. Several strategies were tested to determine their effect on chitosan mediated transfection efficiency: co-administration with polyplexes, co-complexation at the time of polyplex formation, and covalent ligation to chitosan. Our results show that co-complexation and covalent ligation of the NLS peptide derived from IGFBP-3 to chitosan polyplexes yields a 2-fold increase in transfection efficiency, which was not observed for NLS peptide derived from IGFBP-5. These results indicate that the integration of IGFBP-NLS-3 peptides into polyplexes has potential as a strategy to enhance the efficiency of non-viral vectors.pt_PT
dc.description.sponsorshipFCT: PTDC/BTM/ORG/28121/2017; PD/BD/52424/2013; SFRH/BD/76873/2011;PIRG-GA-2009-249314pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1042/BSR20201026pt_PT
dc.identifier.issn0144-8463
dc.identifier.urihttp://hdl.handle.net/10400.1/15393
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPortland Presspt_PT
dc.relationDEVELOPMENT OF NON-VIRAL VECTORS FOR GENE THERAPY FOR PATHOLOGIES OF THE RETINA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleHuman-derived NLS enhance the gene transfer efficiency of chitosanpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDEVELOPMENT OF NON-VIRAL VECTORS FOR GENE THERAPY FOR PATHOLOGIES OF THE RETINA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04462%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-BEB%2F098475%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F70318%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F78404%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.citation.issue1pt_PT
oaire.citation.titleBioscience Reportspt_PT
oaire.citation.volume41pt_PT
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
person.familyNameBitoque
person.familyNameOliveira
person.familyNameCalado
person.familyNameGlória Simão
person.familyNameRosa da Costa
person.givenNameDiogo
person.givenNameAna
person.givenNameSofia
person.givenNameSónia Daniel
person.givenNameAna M
person.identifier2140108
person.identifierhttps://scholar.google.com/citations?hl=pt-PT&user=VLL3QD4AAAAJ&view_op=list_works&sortby=pubdate
person.identifier305029
person.identifier.ciencia-idD81C-6457-7F28
person.identifier.ciencia-idCA17-42CB-F11B
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person.identifier.ciencia-idC612-D462-A564
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.orcid0000-0002-6536-6578
person.identifier.orcid0000-0003-3162-250X
person.identifier.orcid0000-0001-5509-4145
person.identifier.orcid0000-0002-0238-6395
person.identifier.orcid0000-0003-0225-9537
person.identifier.ridK-2202-2016
person.identifier.ridE-2165-2012
person.identifier.scopus-author-id55755464000
person.identifier.scopus-author-id55989431200
person.identifier.scopus-author-id56426215600
person.identifier.scopus-author-id983648
person.identifier.scopus-author-id53986075100
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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