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Recent developments in quantification methods for metallothionein

2002Journal article Restricted access
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dc.contributor.authorDabrio, Marta
dc.contributor.authorRodrı́guez, Adela R
dc.contributor.authorBordin, Guy
dc.contributor.authorBebianno, Maria João
dc.contributor.authorDe Ley, Marc
dc.contributor.authorŠestáková, Ivana
dc.contributor.authorVašák, Milan
dc.contributor.authorNordberg, Monica
dc.date.accessioned2020-05-25T14:37:37Z
dc.date.available2020-05-25T14:37:37Z
dc.date.issued2002
dc.description.abstractThe metallothioneins (MT), a family of proteins with relatively low molecular weight (6-7 kDa), are characterised by the intrinsic presence of 20 cysteinyl groups in their structure, which confers unique metal binding properties to the molecule. Since MT are involved in biological roles, quantification of MT remains an important task. To date, a large number of determination methods have been developed. In this paper recent developments, from 1995 to the present, in methodology employed in quantification studies of total NIT and MT polymorphism are described. Different fields were taken into consideration, such as (i) separation techniques and hyphenated systems, (ii) electrochemical methods, (iii) immunological methods and (iv) quantification of MT mRNA. The data presented are based on our own and published results. A brief overview of the use of metallothionein as a biomarker is included as a relevant example of the importance of MT quantification. Finally, general problems associated with determination and evaluation of obtained results within the above four topics are mentioned.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/S0162-0134(01)00374-9pt_PT
dc.identifier.issn0162-0134
dc.identifier.urihttp://hdl.handle.net/10400.1/13935
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMetallothioneinpt_PT
dc.subjectQuantificationpt_PT
dc.subjectElectrochemistrypt_PT
dc.subjectChromatographypt_PT
dc.subjectImmunochemistrypt_PT
dc.subjectMetalspt_PT
dc.subjectMetallothionein messenger RNApt_PT
dc.subjectCapillary electrophoresis (CE)pt_PT
dc.titleRecent developments in quantification methods for metallothioneinpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage134pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage123pt_PT
oaire.citation.titleJournal of Inorganic Biochemistrypt_PT
oaire.citation.volume88pt_PT
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32

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