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Comparative analysis of a teleost skeleton transcriptome provides insight into its regulation

2013-09-15Journal article Restricted access
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dc.contributor.authorVieira, Florbela A.
dc.contributor.authorThorne, Michael A. S.
dc.contributor.authorStueber, K.
dc.contributor.authorDarias, M.
dc.contributor.authorReinhardt, Richard
dc.contributor.authorClark, M. S.
dc.contributor.authorGisbert, Enric
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-21T14:17:54Z
dc.date.available2014-10-21T14:17:54Z
dc.date.issued2013-09-15
dc.description.abstractAn articulated endoskeleton that is calcified is a unifying innovation of the vertebrates, however the molecular basis of the structural divergence between terrestrial and aquatic vertebrates, such as teleost fish, has not been determined. In the present study long-read next generation sequencing (NGS, Roche 454 platform) was used to characterize acellular perichondral bone (vertebrae) and chondroid bone (gill arch) in the gilthead sea bream (Sparus auratus). A total of 15.97 and 14.53 Mb were produced, respectively from vertebrae and gill arch cDNA libraries and yielded 32,374 and 28,371 contigs (consensus sequences) respectively. 10,455 contigs from vertebrae and 10,625 contigs from gill arches were annotated with gene ontology terms. Comparative analysis of the global transcriptome revealed 4249 unique transcripts in vertebrae, 4201 unique transcripts in the gill arches and 3700 common transcripts. Several core gene networks were conserved between the gilthead sea bream and mammalian skeleton. Transcripts for putative endocrine factors were identified in acellular gilthead sea bream bone suggesting that in common with mammalian bone it can act as an endocrine tissue. The acellular bone of the vertebra, in contrast to current opinion based on histological analysis, was responsive to a short fast and significant (p < 0.05) down-regulation of several transcripts identified by NGS, osteonectin, osteocalcin, cathepsin K and IGFI occurred. In gill arches fasting caused a significant (p < 0.05) down-regulation of osteocalcin and up-regulation of MMP9.por
dc.description.sponsorshipThe authors thank Angela Ramos for extraction of RNA. The study was funded by Lifecycle EU-FP7 222719. FAV was in receipt of a Post-doctoral Grant (BPD/73597/ 2010) from the Science and Technology Foundation (FCT), Portugal. MAST and MSC were funded by Natural Environment Research Council core funding to the British Antarctic Survey.por
dc.identifier.citationFlorbela A. Vieira, M.A.S. Thorne, K. Stueber, M. Darias, R. Reinhardt, M.S. Clark, E. Gisbert, D.M. Power. Comparative analysis of a teleost skeleton transcriptome provides insight into its regulation. General and Comparative Endocrinology 191 (2013) 45–58.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2013.05.025
dc.identifier.issn0016-6480
dc.identifier.otherAUT: DPO00386
dc.identifier.urihttp://hdl.handle.net/10400.1/5391
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0016648013002645por
dc.subjectAdvanced teleostpor
dc.subjectEndocrinepor
dc.subjectNGSpor
dc.subjectTissue responsivenesspor
dc.subjectVertebrae and gill arch transcriptomespor
dc.titleComparative analysis of a teleost skeleton transcriptome provides insight into its regulationpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/222719/EU
oaire.citation.endPage58por
oaire.citation.startPage45por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume191por
oaire.fundingStreamFP7
person.familyNameGisbert
person.familyNamePower
person.givenNameEnric
person.givenNameDeborah Mary
person.identifier606918
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-7457-8468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridB-3654-2011
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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