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Excitation–contraction coupling reflects the metabolic profile of mantle muscle in young cuttlefish

dc.contributor.authorCallaghan, Neal I.
dc.contributor.authorDucros, Loïck
dc.contributor.authorBennett, J. Craig
dc.contributor.authorCapaz, Juan Carlos
dc.contributor.authorAndrade, José Pedro Andrade
dc.contributor.authorSykes, António
dc.contributor.authorDriedzic, William R.
dc.contributor.authorLamarre, Simon G.
dc.contributor.authorMacCormack, Tyson J.
dc.date.accessioned2024-11-08T11:43:38Z
dc.date.available2024-11-08T11:43:38Z
dc.date.issued2024-08-26
dc.description.abstractThe mantle muscle of common cuttlefish, Sepia officinalis, is responsible both for high-magnitude and rapid movements for locomotion, as well as sustained ventilation, which require specific metabolic, electrophysiological, and structural organization. Young cuttlefish have a highly oxidative phenotype and a rapid growth rate. Here, we show high rates of oxygen consumption and protein synthesis in juveniles, and these rates decay exponentially over the first few weeks of growth. This is associated with considerable citrate synthase activity (relative to larger cuttlefish) but a lack of glucose metabolism based on zero uptake of glucose by isolated muscle sheets and minimal activity of hexokinase (similar to larger animals). In contrast to glucose metabolism in the heart, glucose metabolism in these muscle sheets was not stimulated by extracellular taurine. Previous research revealed an unusual ion channel complement in mantle myocytes, the most notable feature of which is the lack of a Na+ current during depolarization. Because this adaptation is not consistent across the coleoid clade, we investigated excitation-contraction coupling. Here, mantle energetics and contractility, including the individual components of the total Ca2+ flux driving contraction, were studied. Results indicate that the majority of Ca2+ current underlying contractile stress development capacity in cuttlefish juveniles is not mediated by dihydropyridine-sensitive L-type channels, in contrast to their adult counterparts, and the sarcoplasmic reticulum contributes little to routine contractility. We had previously noted an influence of physiological levels of taurine in limiting cardiac contractility but found no analogous sensitivity in mantle muscle. Finally, transmission electron microscopy of subcellular architecture revealed the presence of sarcoplasmic tubular aggregates, suggesting that oxidative inhibition of sarcoplasmic reticulum function limits its role in this life stage.eng
dc.description.sponsorship16-02-01-FMP-53; UID/Multi/04326/2016; PPBI-POCI-01-0145-FEDER-022122; ALG-01-0145-FEDER-022121
dc.identifier.doi10.1111/ivb.12439
dc.identifier.eissn1744-7410
dc.identifier.issn1077-8306
dc.identifier.urihttp://hdl.handle.net/10400.1/26234
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relationTowards cephalopod aquaculture: cuttlefish as the spearhead species
dc.relationCentre of Marine Sciences
dc.relationCentre for Biomedical Research
dc.relation.ispartofInvertebrate Biology
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectCalcium flux
dc.subjectExcitation-contraction coupling
dc.subjectGlucose uptake
dc.subjectMetabolic enzymes
dc.subjectMuscle
dc.subjectOxygen consumption
dc.subjectSarcoplasmic reticulum
dc.subjectTaurine
dc.subjectTubular aggregates
dc.titleExcitation–contraction coupling reflects the metabolic profile of mantle muscle in young cuttlefisheng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTowards cephalopod aquaculture: cuttlefish as the spearhead species
oaire.awardTitleCentre of Marine Sciences
oaire.awardTitleCentre for Biomedical Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00576%2F2014%2FCP1217%2FCT0002/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIM%2F04773%2F2013/PT
oaire.citation.issue3
oaire.citation.startPagee12439
oaire.citation.titleInvertebrate Biology
oaire.citation.volume143
oaire.fundingStreamInvestigador FCT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCapaz
person.familyNameAndrade
person.familyNameSykes
person.givenNameJuan Carlos
person.givenNameJosé Pedro Andrade
person.givenNameAntónio
person.identifier107454
person.identifier.ciencia-id5817-4C54-7E98
person.identifier.ciencia-id7510-6641-5A42
person.identifier.orcid0000-0003-4682-5756
person.identifier.orcid0000-0002-7816-1707
person.identifier.orcid0000-0002-5207-0612
person.identifier.ridC-3609-2012
person.identifier.scopus-author-id57194230202
person.identifier.scopus-author-id7102626426
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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