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Identification of a new cartilage-specific S100-like protein up-regulated during endo/perichondral mineralization in gilthead seabream

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dc.contributor.authorFonseca, V. G.
dc.contributor.authorRosa, Joana
dc.contributor.authorLaizé, Vincent
dc.contributor.authorGavaia, Paulo J.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-04T12:18:31Z
dc.date.available2014-06-04T12:18:31Z
dc.date.issued2011
dc.date.updated2014-05-30T10:01:43Z
dc.description.abstractCalcium ions and calcium-binding proteins play a major role in many cellular processes, in particular skeletogenesis and bone formation. We report here the discovery of a novel S100 protein in fish and the analysis of its gene expression patterns. A 648-bp full-length cDNA encoding an 86-amino acid S100-like calcium-binding protein was identified through the subtractive hybridization of a gilthead seabream (Sparus aurata) cDNA library constructed to identify genes associated with in vitro mineralization. Deduced protein lacks an identifiable signal peptide and exhibits two EF-hand motifs characteristic of S100 proteins. Phylogenetic and bioinformatic analyses of S100 sequences suggested that gilthead seabream protein represents a novel and fish-specific member of the S100 protein family. Expression of S100-like gene was up-regulated during the in vitro mineralization of bone-derived cell lines and during seabream development, from larvae throughout adulthood, reflecting skeletogenesis. Restriction of S100-like gene expression to chondrocytes of cartilaginous tissues undergoing endo/perichondral mineralization in juvenile fish further confirmed the mineralogenic role of the protein in fish and emphasized the potential of S100-like as a marker of mineralizing cartilage in developing fish.por
dc.identifier.citationFonseca, V.G.; Rosa, J.; Laizé, V.; Gavaia, P.J.; Cancela, M.L. Identification of a new cartilage-specific S100-like protein up-regulated during endo/perichondral mineralization in gilthead seabream, Gene Expression Patterns, 11, 7, 448-455, 2011.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.gep.2011.07.003
dc.identifier.issn1567-133X
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4178
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectGilthead seabream Sparus aurata L.por
dc.subjectTeleosteipor
dc.subjectBone-derived cell linepor
dc.subjectTissue mineralizationpor
dc.subjectCalcium-binding proteinpor
dc.subjectSubtractive librarypor
dc.titleIdentification of a new cartilage-specific S100-like protein up-regulated during endo/perichondral mineralization in gilthead seabreampor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage455por
oaire.citation.issue7por
oaire.citation.startPage448por
oaire.citation.titleGene Expression Patternspor
oaire.citation.volume11por
person.familyNameRosa
person.familyNameLaizé
person.familyNameGavaia
person.familyNameCancela
person.givenNameJoana
person.givenNameVincent
person.givenNamePaulo
person.givenNameM. Leonor
person.identifier.ciencia-id0B18-8AEC-875C
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0001-7947-2681
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridB-4463-2008
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id36505067900
person.identifier.scopus-author-id6602982778
person.identifier.scopus-author-id6507104377
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication181f1017-5086-40dc-9edc-a289aaa2d263
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublication9dca2139-21a4-4d59-aaf7-531f1033a58e
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscovery181f1017-5086-40dc-9edc-a289aaa2d263

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