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Fluorescent dye nano-assemblies by thiol attachment directed to the tips of gold nanorods for effective emission enhancement

2020-03-19Journal article Restricted access
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dc.contributor.authorBotequim, David
dc.contributor.authorSilva, Inês I. R.
dc.contributor.authorSerra, Sofia G.
dc.contributor.authorEP, Melo
dc.contributor.authorPrazeres, Duarte M. F.
dc.contributor.authorCosta, Sílvia M. B.
dc.contributor.authorPaulo, Pedro M. R.
dc.date.accessioned2020-07-28T09:51:32Z
dc.date.available2020-07-28T09:51:32Z
dc.date.issued2020-03-19
dc.description.abstractThe conjugation of dye-labelled DNA oligonucleotides with gold nanorods has been widely explored for the development of multifunctional fluorescent nanoprobes. Here, we show that the functionalization route is crucial to achieve enhanced emission in dye nano-assemblies based on gold nanorods. By using a tip-selective approach for thiol attachment of dye molecules onto gold nanorods, it was possible to effectively increase the emission by more than 10-fold relatively to that of a free dye. On the other hand, a non-selective approach revealed that indiscriminate surface functionalization has a detrimental effect on the enhancement. Simulations of discrete dipole approximation gave further insight into the surface distribution of plasmon-enhanced emission by confirming that tip regions afford an effective enhancement, while side regions exhibit a negligible effect or even emission quenching. The contrast between dye nano-assemblies obtained from tip- and non-selective functionalization was further characterized by single-particle fluorescence emission. These studies showed that tip-functionalized gold nanorods with an average of only 30 dye molecules have a comparable to or even stronger emission than non-selectively functionalized particles with approximately 10 times more dye molecules. The results herein reported could significantly improve the performance of dye nano-assemblies for imaging or sensing applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1039/d0nr00267dpt_PT
dc.identifier.issn2040-3364
dc.identifier.urihttp://hdl.handle.net/10400.1/14528
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relationInstitute for Bioengineering and Biosciences
dc.relationPlasmonic Enhancement of Fluorescence Signalling for Pushing the Limit of Micro-RNA Detection
dc.subjectSingle molecule flourescencept_PT
dc.subjectNanoparticlespt_PT
dc.titleFluorescent dye nano-assemblies by thiol attachment directed to the tips of gold nanorods for effective emission enhancementpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardTitlePlasmonic Enhancement of Fluorescence Signalling for Pushing the Limit of Micro-RNA Detection
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIO%2F04565%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBPD%2F111906%2F2015/PT
oaire.citation.endPage6345pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage6334pt_PT
oaire.citation.titleNanoscalept_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNamePinho Melo
person.givenNameEduardo
person.identifier1443188
person.identifier.ciencia-id3C1C-C10C-1510
person.identifier.orcid0000-0002-0974-8977
person.identifier.scopus-author-id35566177900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication5fa1895f-5577-4652-961a-886ec9bf41b1
relation.isAuthorOfPublication.latestForDiscovery5fa1895f-5577-4652-961a-886ec9bf41b1
relation.isProjectOfPublication1244bed0-e024-4e42-b9f3-8a88b969ad15
relation.isProjectOfPublicationa30c46fb-56b8-4549-8ab4-8d82b863cd8a
relation.isProjectOfPublication.latestForDiscovery1244bed0-e024-4e42-b9f3-8a88b969ad15

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