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IR exciton activation mechanism of ethanol oxidation by human alcohol dehydrogenase (ADH) 1A enzyme

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dc.contributor.authorKhmelinskii, Igor
dc.contributor.authorMakarov, Vladimir I.
dc.date.accessioned2023-09-19T13:56:05Z
dc.date.available2023-09-19T13:56:05Z
dc.date.issued2023
dc.description.abstractLow-energy (2500 cm(-1)) exciton transfer was explored in Muller cell (MC) intermediate filaments (IFs) isolated from porcine retina and filling a capillary matrix. Excitons were generated by absorption of IR radiation at 4 mu m. The effects of these excitons on ethanol oxidation by human alcohol dehydrogenase (ADH1A) enzyme were quantified. It was found that IR excitons transferred to the enzyme accelerated alcohol oxidation rate, which increased by the factor of 2.76 when exciting the IFs with 2.39 mu W/cm(2) of 4 mu m IR light. Power dependence of the oxidation rate was also explored. These results show that IFs may be transmitting energy to enzyme mole-cules in vivo, facilitating enzymatic reactions. The required excitons may be produced by cells at the cost of adenosine triphosphate (ATP) hydrolysis energy. Therefore, such control mechanism for enzymatic reactions may be operational in living systems. Direct activation of the enzyme by IR radiation with 4 mu m wavelength did not occur; instead, indirect activation of the IF...ADH1A...NAD (nicotinamide adenine dinucleotide)...EtOH complex occurred by energy transfer of the IR exciton to the ADH1A molecule of this complex. Considering that every living cell has a network of IFs, a similar reaction control mechanism may be operational in vivo, providing a much faster energy supply redirection within the cell than ATP diffusion, and justifying a closer inquiry.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jphotochem.2022.114371pt_PT
dc.identifier.issn1010-6030
dc.identifier.urihttp://hdl.handle.net/10400.1/20004
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectIntermediate filamentspt_PT
dc.subjectEnergy guidept_PT
dc.subjectHuman alcohol dehydrogenase ADH1Apt_PT
dc.subjectLow-energy electronic excited statespt_PT
dc.subjectIR exciton transfer to enzymept_PT
dc.titleIR exciton activation mechanism of ethanol oxidation by human alcohol dehydrogenase (ADH) 1A enzymept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage114371pt_PT
oaire.citation.titleJournal of Photochemistry and Photobiology A: Chemistrypt_PT
oaire.citation.volume436pt_PT
person.familyNameKhmelinskii
person.givenNameIgor
person.identifier0000000420541031
person.identifier.ciencia-id0D1A-CB6C-6316
person.identifier.orcid0000-0002-6116-184X
person.identifier.ridC-9587-2011
person.identifier.scopus-author-id6701444934
rcaap.contributor.authoremailikhmelin@ualg.ptpt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationfcb9f09f-2e99-41fb-8c08-7e1acbc65076
relation.isAuthorOfPublication.latestForDiscoveryfcb9f09f-2e99-41fb-8c08-7e1acbc65076

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