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Temperature effects on growth, metabolome, lipidic profile and photosynthetic pigment content of Microglena antarctica (chlorophyceae): a comprehensive analysis

2024Journal article Open access
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dc.contributor.authorTrentin, Riccardo
dc.contributor.authorMoschin, Emanuela
dc.contributor.authorCustódio, Luísa
dc.contributor.authorMoro, Isabella
dc.date.accessioned2024-05-03T09:16:27Z
dc.date.available2024-05-03T09:16:27Z
dc.date.issued2024
dc.description.abstractAntarctic microalgae have evolved a wide range of adaptations to survive at extreme environmental conditions. This study aimed to explore the physiological and biochemical processes occurring in Microglena antarctica (Chlorophyceae) in response to changes in temperature. M. antarctica cultivated at three distinct temperatures (4 degrees C, 8 degrees C and 16 degrees C) exhibited variations in growth patterns, metabolomes, fatty acid methyl esters (FAMEs) profile and photosynthetic pigment concentrations. Our results highlighted a decrease in growth at 16 degrees C, confirming the cryophilic nature of this species. The growth rates at the exponential phase were observed to decrease progressively with an initial rate of (0.29 +/- 0.05 d(-1)) at the culturing temperature of 4 degrees C, followed by 8 degrees C (0.24 +/- 0.09 d(-1)), and further reduction at 16 degrees C (0.16 +/- 0.05 d(-1)). An integrative untargeted metabolomics approach combining mass spectral libraries and novel in-silico tools was employed to improve feature annotation and to provide additional information on features chemical classes. Significant differences in M. antarctica annotated compounds, chemical classes and whole metabolomes were observed among 4, 8 and 16 degrees C. Finally, targeted analyses were performed to evaluate changes in lipid profiles and photosynthetic pigment content. Higher percentages of polyunsaturated fatty acids (PUFAs) were observed at 4 and 8 degrees C, approximately 65.00 % of total FAMEs, and decreased to 60.71 % at 16 degrees C. Monounsaturated fatty acids (MUFAs) significantly increased at 16 degrees C, reaching up to 10.96 % of total FAMEs, in contrast to 4 degrees C and 8 degrees C, where the content of MUFAs was around 5.00 %. Additionally, chlorophyll a and carotenoid content increased by 50-100 % at 16 degrees C compared to lower temperatures. The present work highlights temperature-related responses in M. antarctica biochemical profile, combining untargeted and targeted approaches, and physiology, by growth analysis.pt_PT
dc.description.sponsorshipPNRA16_00120 - A1; PNRA18_00078 - B2pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.algal.2024.103461pt_PT
dc.identifier.issn2211-9264
dc.identifier.urihttp://hdl.handle.net/10400.1/20697
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntarctic microalgaept_PT
dc.subjectUntargeted metabolomicspt_PT
dc.subjectFatty acids profilept_PT
dc.subjectPhotosynthetic pigment contentpt_PT
dc.subjectGrowth curvespt_PT
dc.subjectTemperature stresspt_PT
dc.titleTemperature effects on growth, metabolome, lipidic profile and photosynthetic pigment content of Microglena antarctica (chlorophyceae): a comprehensive analysispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F00425%2F2017%2FCP1391%2FCT0001/PT
oaire.citation.startPage103461pt_PT
oaire.citation.titleAlgal Researchpt_PT
oaire.citation.volume79pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2017
person.familyNameCustódio
person.givenNameLuísa
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.orcid0000-0003-4338-7703
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id15831018900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoveryf9cfed0f-6b67-413e-988c-ac7397183471
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