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Transcriptomic insights into metabolic reprogramming and exopolysaccharide synthesis in porphyridium purpureum under gradual nitrogen deprivation

2026-01-13Artigo científico Acesso aberto
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datacite.subject.sdg02:Erradicar a Fome
datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
dc.contributor.authorGUERREIRO, Maurean
dc.contributor.authorEmmanuel, Coline
dc.contributor.authorDupuits, Céline
dc.contributor.authorGardarin, Christine
dc.contributor.authorMouzeyar, Said
dc.contributor.authorVarela, João
dc.contributor.authorRoche, Jane
dc.contributor.authorlaroche, céline
dc.date.accessioned2026-02-19T13:37:06Z
dc.date.available2026-02-19T13:37:06Z
dc.date.issued2026-01-13
dc.description.abstractPorphyridium species are known red microalgae for producing valuable bioactive compounds such as sulfated exopolysaccharides (EPS) with diverse industrial biomedical applications due to their functional and rheological properties. Recent studies have investigated how abiotic stresses, particularly nitrogen deprivation, affect Porphyridium’s metabolic regulation and EPS production through transcriptomic analysis. Still, the mechanisms governing EPS biosynthesis and the involvement of carbohydrate-activated enzymes (CAZymes) remain poorly understood. This study investigated the progressive effects of nitrate consumption on the unicellular red alga, P. purpureum, by integrating physiological, biochemical, and transcriptomic analyses through RNA-Seq, further validated by RT-qPCR. P. purpureum displayed a gradual, phase-dependent metabolic response to progressive nitrogen stress. EPS release coincided with the decline in nitrate uptake, linking nitrogen availability to carbon redirection towards polysaccharide secretion. Transcriptomic data revealed global metabolic downregulation with targeted upregulation of stress-responsive, carbohydrate catabolic, and nucleotide–sugar synthesis pathways, including the upregulation of CAZyme families GT4, GT8, and GT77. Our results give insights into the coordinated nitrogen and carbon metabolic regulation underlying polysaccharide biosynthesis, while opening future perspectives on enzyme compartmentalization and regulatory flux distribution under nitrogen stress in P. purpureum.eng
dc.description.sponsorshipANR- 20-DFRI-0003
dc.identifier.doi10.3390/md24010040
dc.identifier.issn1660-3397
dc.identifier.urihttp://hdl.handle.net/10400.1/28195
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofMarine Drugs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPorphyridium
dc.subjectPolysaccharides
dc.subjectNitrogen
dc.subjectDeprivation
dc.subjectTranscriptomics
dc.titleTranscriptomic insights into metabolic reprogramming and exopolysaccharide synthesis in porphyridium purpureum under gradual nitrogen deprivationeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.issue1
oaire.citation.titleMarine drugs
oaire.citation.volume24
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameGUERREIRO
person.familyNameVarela
person.familyNamelaroche
person.givenNameMaurean
person.givenNameJoão
person.givenNamecéline
person.identifier.orcid0000-0003-3449-2237
person.identifier.orcid0000-0003-3101-693X
person.identifier.orcid0000-0003-2705-2866
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication81e2f293-f3c6-487a-9bc0-277878da4c36
relation.isAuthorOfPublicationd339a9e7-bc32-49c4-ba48-a17343d40092
relation.isAuthorOfPublication07660aa5-acc3-4925-bfe2-26b14acff6cc
relation.isAuthorOfPublication.latestForDiscoveryd339a9e7-bc32-49c4-ba48-a17343d40092
relation.isProjectOfPublicationfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f
relation.isProjectOfPublication794d4c77-c731-471e-bc96-5a41dcd3d872
relation.isProjectOfPublication.latestForDiscoveryfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f

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