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Evolution of the glucagon-like system across fish

2018Journal article Open access
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dc.contributor.authorCardoso, João CR
dc.contributor.authorFélix, Rute C.
dc.contributor.authorCosta, Carina
dc.contributor.authorPFS, Palma
dc.contributor.authorCanario, Adelino
dc.contributor.authorPower, Deborah
dc.date.accessioned2020-08-03T14:09:24Z
dc.date.available2020-08-03T14:09:24Z
dc.date.issued2018
dc.description.abstractIn fishes, including the jawless lampreys, the most ancient lineage of extant vertebrates, plasma glucose levels are highly variable and regulation is more relaxed than in mammals. The regulation of glucose and lipid in fishes in common with mammals involves members of the glucagon (GCG)-like family of gastrointestinal peptides. In mammals, four peptides GCG, glucagon-like peptide 1 and 2 (GLP1 and GLP2) and glucose-dependent insulinotropic peptide (GIP) that activate four specific receptors exist. However, in lamprey and other fishes the glucagon-like family evolved differently and they retained additional gene family members (glucagon-related peptide, gcrp and its receptor, gcrpr) that are absent from mammals. In the present study, we analysed the evolution of the glucagon-like system in fish and characterized gene expression of the family members in the European sea bass (Dicentrarchus labrax) a teleost fish. Phylogenetic analysis revealed that multiple receptors and peptides of the glucagon-like family emerged early during the vertebrate radiation and evolved via lineage specific events. Synteny analysis suggested that family member gene loss is likely to be the result of a single gene deletion event. Lamprey was the only fish where a putative glp1r persisted and the presence of the receptor gene in the genomes of the elephant shark and coelacanth remains unresolved. In the coelacanth and elephant shark, unique proglucagon genes were acquired which in the former only encoded Gcg and Glp2 and in the latter, shared a similar structure to the teleost proglucagon gene but possessed an extra exon coding for Glp-like peptide that was most similar to Glp2. The variable tissue distribution of the gene transcripts encoding the ligands and receptors of the glucagon-like system in an advanced teleost, the European sea bass, suggested that, as occurs in mammals, they have acquired distinct functions. Statistically significant (p < .05) down-regulation of teleost proglucagon a in sea bass with modified plasma glucose levels confirmed the link between these peptides and metabolism. The tissue distribution of members of the glucagon-like system in sea bass and human suggests that evolution of the brain-gut-peptide regulatory loop diverged between teleosts and mammals despite the overall conservation and similarity of glucagon-like family members.pt_PT
dc.description.sponsorshipFCT: SFRH/BD/103185/2014pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ygcen.2017.10.003pt_PT
dc.identifier.issn0016-6480
dc.identifier.urihttp://hdl.handle.net/10400.1/14582
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAcademic Presspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAmino acid sequencept_PT
dc.subjectAnimalspt_PT
dc.subjectFishespt_PT
dc.subjectGene expression profilingpt_PT
dc.subjectGenomept_PT
dc.subjectGlucagonpt_PT
dc.subjectHumanspt_PT
dc.subjectPeptidespt_PT
dc.subjectPhylogenypt_PT
dc.subjectReceptorspt_PT
dc.subjectSyntenypt_PT
dc.subjectEvolutionpt_PT
dc.subjectMolecularpt_PT
dc.subjectCell surfacept_PT
dc.titleEvolution of the glucagon-like system across fishpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F121279%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F89811%2F2012/PT
oaire.citation.endPage130pt_PT
oaire.citation.startPage113pt_PT
oaire.citation.titleGeneral and Comparative Endocrinologypt_PT
oaire.citation.volume264pt_PT
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameCardoso
person.familyNameFélix
person.familyNamePedrosa da Costa
person.familyNamePalma
person.familyNameCanario
person.familyNamePower
person.givenNameJoão
person.givenNameRute
person.givenNameCarina Filipa
person.givenNamePedro
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifier14332
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person.identifier.orcid0000-0001-7890-0170
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person.identifier.orcid0000-0002-9861-4363
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-4151-2013
person.identifier.ridM-6164-2013
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id56006773000
person.identifier.scopus-author-id56365905500
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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