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Variation of metal and metallothionein concentrations in a natural population of ruditapes decussatus

2003Journal article Restricted access
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dc.contributor.authorBebianno, Maria João
dc.contributor.authorSerafim, A.
dc.date.accessioned2020-05-25T10:06:05Z
dc.date.available2020-05-25T10:06:05Z
dc.date.issued2003
dc.description.abstractThe spatial and seasonal variation of total and subcellular distribution of Cd, Cu, and Zn was followed in different tissues (gills, digestive gland, and remaining tissues) of the clam Ruditapes decussatus collected along a metal contamination gradient in the Ria Formosa lagoon (southern Portugal) and compared with metallothionein (MT) concentrations.Total metal concentrations decreased according to the sequence digestive gland > gills > remaining tissues for Cd, digestive gland approximately gills > remaining tissues for Cu and gills > digestive gland > remaining tissues for Zn. MT concentrations in these tissues decreased according to the same sequence observed for Cd. In all the tissues, the highest subcellular concentration was in the cytosol for Cd and Cu and in the pellet for Zn. Among the three metals, Cd concentrations showed the most evident spatial variation. In all tissues, total and subcellular Cd concentrations decreased from the inner parts of the lagoon toward the ocean. However, no significant spatial or seasonal variation occurred in clam tissues for the other two metals, though marginal elevated Cu concentrations were observed in the inner parts of the lagoon. Therefore, Cu subcellular distribution in clam tissues was not significantly altered by Cu changes in the lagoon and are the baseline levels for normal metabolism of this clam population. The fact that total Zn concentrations remained unchanged both spatial and seasonal suggested that these clams regulate Zn in their tissues. In the three tissues, MT bind most significantly to Cd and Cu, while Zn, although binding to MT, is preferably bound to other ligands. MT concentrations showed the same spatial and seasonal variation of Cd and were significantly related with total and heat-treated cytosolic Cd in all tissues. For Cu a significant relationship between MT and total or cytosolic Cu was only observed in the remaining tissues. No relationship was observed between MT and total or cytosolic Zn concentrations. Metals and MT concentrations increased with the increase in the condition index for the gills and the digestive gland and decreased from the remaining tissues.Cd concentrations in the gills increased only in the heat-treated cytosolic fraction while Zn in this fraction decreased. Thus Cd concentrations in this tissue displaced Zn from the MT-fraction, leading to a modification of the soluble/insoluble Zn ratio once total Zn concentrations remained unchanged. This modification reflects a perturbation in the normal metabolism in this tissue due to the excess of Cd present. With the exception of the gills, Zn subcellular distribution in the other two tissues was similar among sites and season. The model that describes the relationship between MT, metals, and weight in the gills, digestive gland and remaining tissues also indicates that Cd was the only metal that influence MT synthesis significantly in all the tissues. The induced and/or existent MT was sufficient to bind free Cd ions present in the cells, preventing any damage to cellular metabolism in this clam population. Therefore, MT in the gills and digestive gland of R. decussatus can be used as an early warning signal for Cd exposure and are a useful biomarker to assess the toxicological status of this population in the Ria Formosa lagoon.pt_PT
dc.description.sponsorshipPortuguese Foundation of Science and Technology: grant BD/254/93-IG and project STRDB/C/AMB/32/92pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00244-002-2004-7pt_PT
dc.identifier.issn0090-4341
dc.identifier.urihttp://hdl.handle.net/10400.1/13930
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectBiomarkerspt_PT
dc.subjectBivalviapt_PT
dc.subjectCadmiumpt_PT
dc.subjectCopperpt_PT
dc.subjectCytosolpt_PT
dc.subjectGillspt_PT
dc.subjectLigandspt_PT
dc.subjectMetallothioneinpt_PT
dc.subjectSeasonspt_PT
dc.subjectTissue distributionpt_PT
dc.subjectWater pollutantspt_PT
dc.subjectZincpt_PT
dc.titleVariation of metal and metallothionein concentrations in a natural population of ruditapes decussatuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage66pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage53pt_PT
oaire.citation.titleArchives of Environmental Contamination and Toxicologypt_PT
oaire.citation.volume44pt_PT
person.familyNameBebianno
person.familyNamePereira Serafim
person.givenNameMaria
person.givenNameMaria Angela
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.ciencia-id9C18-090A-5E3D
person.identifier.orcid0000-0003-1492-8566
person.identifier.orcid0000-0002-1165-0645
person.identifier.scopus-author-id7004152715
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication0e42fc03-7052-438d-952b-b3caa5bdc36a
relation.isAuthorOfPublication.latestForDiscovery0e42fc03-7052-438d-952b-b3caa5bdc36a

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