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Molecular and functional study of AQY1 from Saccharomyces cerevisiae: role of the C-terminal domain

1999-04-02Journal article Restricted access
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dc.contributor.authorLaizé, Vincent
dc.contributor.authorGobin, R.
dc.contributor.authorRousselet, G.
dc.contributor.authorBadier, C.
dc.contributor.authorHohmann, S.
dc.contributor.authorRipoche, P.
dc.contributor.authorTacnet, F.
dc.date.accessioned2014-06-05T14:03:01Z
dc.date.available2014-06-05T14:03:01Z
dc.date.issued1999-04-02
dc.date.updated2014-06-03T10:27:37Z
dc.description.abstractThe yeast YPR192w gene, which encodes a protein (Aqy1p) with strong homology to aquaporins (AQPs), was cloned from nine S. cerevisiae strains. The osmotic water permeability coefficient (Pf) of X. laevis oocytes expressing the gene cloned from the S1278b strain (AQY1-1) was 5.7 times higher than the Pf of oocytes expressing the gene cloned from other strains (AQY1-2). Aqy1-1p, initially cloned without its C-terminus (Aqy1-1DCp), mediated an ;3 times higher water permeability than the full-length protein. This corresponds to a 3-fold higher protein density in the oocyte plasma membrane, as shown by freeze-fracture electron microscopy. Pf measurements in yeast spheroplasts confirmed the presence of functional water channels in S1278b and a pharmacological study indicated that this strain contains at least a second functional aquaporin.por
dc.identifier.citationLaizé V; Gobin R; Rousselet G; Badier C; Hohmann S; Ripoche P; Tacnet F. Molecular and functional study of AQY1 from Saccharomyces cerevisiae: role of the C-terminal domain, Biochemical and Biophysical Research Communications, 257, 1, 139-144, 1999.por
dc.identifier.doihttp://dx.doi.org/ http://dx.doi.org/10.1006/bbrc.1999.0425
dc.identifier.issn0006-291X
dc.identifier.urihttp://hdl.handle.net/10400.1/4204
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0006291X99904254por
dc.subjectWater transportpor
dc.subjectGenetic heterogeneitypor
dc.subjectSaccharomyces cerevisiaepor
dc.subjectAquaporinpor
dc.subjectAQY1por
dc.titleMolecular and functional study of AQY1 from Saccharomyces cerevisiae: role of the C-terminal domainpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage144por
oaire.citation.issue1por
oaire.citation.startPage139por
oaire.citation.titleBiochemical and Biophysical Research Communicationspor
oaire.citation.volume257por
person.familyNameLaizé
person.givenNameVincent
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id6602982778
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublication.latestForDiscoveryb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f

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