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Microbiome matters: how transplantation methods and donor origins shape the successful restoration of the seagrass Posidonia oceanica

2025-08-06Journal article Open access
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dc.contributor.authorBoulenger, Arnaud
dc.contributor.authorAires, Tania
dc.contributor.authorEngelen, Aschwin
dc.contributor.authorMuyzer, Gerard
dc.contributor.authorMarengo, Michel
dc.contributor.authorGobert, Sylvie
dc.date.accessioned2025-09-17T09:11:59Z
dc.date.available2025-09-17T09:11:59Z
dc.date.issued2025-08-06
dc.description.abstractBackground Posidonia oceanica forms extensive seagrass meadows in the Mediterranean Sea, providing key ecosystem services. However, these meadows decline due to anthropogenic pressures like anchoring and coastal development. Transplantation-based restoration has been explored for decades, yet the role of the plant-associated microbiome in restoration success remains largely unknown. Results 16 S rRNA gene amplicon sequencing was used to investigate how different transplantation methods and donor origins influence the bacterial communities of P. oceanica cuttings two years post-transplantation. We tested three transplantation methods, iron staples, coconut fiber mats, and BESE elements, and compared them with control meadows and donor populations from two different origins: naturally uprooted storm-fragments and intermatte cuttings manually harvested from established meadows. Our results show that transplantation methods strongly shape bacterial communities in seagrass roots. Iron staples promoted microbial assemblages most similar to natural meadows, likely due to direct sediment contact enhancing recruitment of key functional bacterial orders such as Chromatiales and Desulfobacterales. In contrast, BESE elements and coconut fiber mats displayed dissimilar bacterial communities compared to control meadows, likely due to material composition and physical separation between the cuttings and the sediment. Donor origin had only subtle effects on bacterial communities’ structure, although intermatte cuttings showed higher abundances of Candidatus Thiodiazotropha, a genus thought to be involved sulfur oxidation and nitrogen fixation. Conclusion Our results demonstrate that transplantation methods strongly influence root-associated bacterial communities. Limited sediment contact in elevated substrates delayed the establishment of key functional bacteria, highlighting the importance of direct interaction with the sediment microbial pool. These results imply that restoration strategies should prioritize methods enhancing sediment–root interactions to support microbial recovery. Incorporating microbiome considerations, such as optimized substrates or microbial inoculation, could improve the resilience and long-term success of P. oceanica restoration.eng
dc.description.sponsorshipCCMAR/ ID/16/2018
dc.identifier.doi10.1186/s40793-025-00764-9
dc.identifier.issn2524-6372
dc.identifier.urihttp://hdl.handle.net/10400.1/27698
dc.language.isoeng
dc.peerreviewedyes
dc.publisherBioMed Central
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofEnvironmental Microbiome
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectHolobiont
dc.subjectMicrobiome
dc.subjectRhizosphere
dc.subjectRestoration
dc.subjectSeagrass
dc.subjectSymbiosis
dc.subjectTransplantation
dc.titleMicrobiome matters: how transplantation methods and donor origins shape the successful restoration of the seagrass Posidonia oceanicaeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.issue1
oaire.citation.titleEnvironmental Microbiome
oaire.citation.volume20
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAires
person.familyNameEngelen
person.givenNameTania
person.givenNameAschwin
person.identifier282868
person.identifier.ciencia-id4318-9E2C-32B9
person.identifier.ciencia-id911A-9A0C-744D
person.identifier.orcid0000-0002-1964-6819
person.identifier.orcid0000-0002-9579-9606
person.identifier.ridM-8306-2013
person.identifier.ridM-3432-2013
person.identifier.scopus-author-id6701622770
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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