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First evidence of dermo-protective activity of marine sulfur-containing histidine compounds

2022-11Journal article Restricted access
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dc.contributor.authorBrancaccio, Mariarita
dc.contributor.authorMilito, Alfonsina
dc.contributor.authorS B Viegas, Carla
dc.contributor.authorPalumbo, Anna
dc.contributor.authorSimes, Dina
dc.contributor.authorCastellano, Immacolata
dc.date.accessioned2023-01-20T15:48:58Z
dc.date.available2023-01-20T15:48:58Z
dc.date.issued2022-11
dc.description.abstractAmong natural products, ovothiol (ovo), produced by marine invertebrates, bacteria, and microalgae, is receiving increasing interest for its unique antioxidant properties. Recently, ovo has been shown to exhibit anti-inflammatory activity in an in vitro model of endothelial dysfunction and in an in vivo model of liver fibrosis.The aim of this study was to evaluate the effect of ovo and its precursor 5-thiohistidine (5-thio) in comparison with ergothioneine (erg), in human skin cells and tissues upon inflammation. We used both an in vitro and ex vivo model of human skin, represented by a keratinocytes cell line (HaCaT) and skin biopsies, respectively. We observed that ovo, 5-thio, and erg were not cytotoxic in HaCaT cells, but instead exerted a protective function against TNF-alpha-induced inflammation. In order to get insights on their mechanism of action, we performed western blot analysis of ERK and JNK, as well as sub-cellular localization of Nrf2, a key mediator of the anti-inflammatory response. The results indicated that the pre-treatment with ovo, 5-thio, and erg differently affected the phosphorylation of ERK and JNK. However, all the three molecules promoted the accumulation of Nrf2 in the nucleus of HaCaT cells. In addition, gene expression analysis by RTqPCR and ELISA assays performed in ex vivo human skin tissues pre-treated with thiohistidines and then inflamed with IL-1 beta revealed a significant downregulation of IL-8, TNF-alpha and COX-2 genes and a concomitant significant decrease in the cytokines IL-6, IL-8 and TNF-alpha production. Moreover, the protective action of ovo and 5-thio resulted to be stronger when compared with dexamethasone, a corticosteroid drug currently used to treat skin inflammatory conditions.Our findings suggest that ovo and 5-thio can ameliorate skin damage and may be used to develop natural skin care products to prevent the inflammatory status induced by environmental stressors and aging.pt_PT
dc.description.sponsorshipDL57/2016/CP1361/CT0006
dc.description.sponsorshipLA/P/0101/2020
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.freeradbiomed.2022.09.017pt_PT
dc.identifier.eissn1873-4596
dc.identifier.urihttp://hdl.handle.net/10400.1/18883
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationEuropean Marine Biological Research Infrastructure Cluster to promote the Blue Bioeconomy
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.subjectOvothiolpt_PT
dc.subjectThiohistidinept_PT
dc.subjectErgothioneinept_PT
dc.subjectMarine antioxidantspt_PT
dc.subjectInflammationpt_PT
dc.subjectSkinpt_PT
dc.subjectKeratinocytespt_PT
dc.titleFirst evidence of dermo-protective activity of marine sulfur-containing histidine compoundspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEuropean Marine Biological Research Infrastructure Cluster to promote the Blue Bioeconomy
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/654008/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.citation.endPage234pt_PT
oaire.citation.startPage224pt_PT
oaire.citation.titleFree Radical Biology and Medicinept_PT
oaire.citation.volume192pt_PT
oaire.fundingStreamH2020
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameViegas
person.familyNameSimes
person.givenNameCarla
person.givenNameDina
person.identifier.ciencia-idC414-F596-EEC6
person.identifier.orcid0000-0002-5765-3665
person.identifier.orcid0000-0002-5145-4753
person.identifier.ridN-6695-2014
person.identifier.ridN-2789-2014
person.identifier.scopus-author-id8656310300
person.identifier.scopus-author-id7201884723
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication33984b3c-ffac-477a-896a-ddbf7aced4e3
relation.isAuthorOfPublication31bac96d-1d01-4b8f-8f2b-9da4be31ea41
relation.isAuthorOfPublication.latestForDiscovery31bac96d-1d01-4b8f-8f2b-9da4be31ea41
relation.isProjectOfPublicationd5cd89fe-f500-412a-ab83-8d5c84eed8b1
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