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Molecular Taxonomic Profiling of Bacterial Communities in a Gilthead Seabream (Sparus aurata) Hatchery

2017-02Journal article Open Access
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dc.contributor.authorCalifano, Gianmaria
dc.contributor.authorCastanho, Sara
dc.contributor.authorSoares, Florbela
dc.contributor.authorRibeiro, Laura
dc.contributor.authorCox, C. J.
dc.contributor.authorMata, Leonardo
dc.contributor.authorCosta, Rodrigo
dc.date.accessioned2018-12-07T14:53:01Z
dc.date.available2018-12-07T14:53:01Z
dc.date.issued2017-02
dc.description.abstractAs wild fish stocks decline worldwide, land-based fish rearing is likely to be of increasing relevance to feeding future human generations. Little is known about the structure and role of microbial communities in fish aquaculture, particularly at larval developmental stages where the fish microbiome develops and host animals are most susceptible to disease. We employed next-generation sequencing (NGS) of 16S rRNA gene reads amplified from total community DNA to reveal the structure of bacterial communities in a gilthead seabream (Sparus aurata) larviculture system. Early-(2 days after hatching) and late-stage (34 days after hatching) fish larvae presented remarkably divergent bacterial consortia, with the genera Pseudoalteromonas, Marinomonas, Acinetobacter, and Acidocella (besides several unclassified Alphaproteobacteria) dominating the former, and Actinobacillus, Streptococcus, Massilia, Paracoccus, and Pseudomonas being prevalent in the latter. A significant reduction in rearing-water bacterial diversity was observed during the larviculture trial, characterized by higher abundance of the Cryomorphaceae family (Bacteroidetes), known to populate microniches with high organic load, in late-stage rearing water in comparison with early-stage rearing-water. Furthermore, we observed the recruitment, into host tissues, of several bacterial phylotypes-including putative pathogens as well as mutualists-that were detected at negligible densities in rearing-water or in the live feed (i.e., rotifers and artemia). These results suggest that, besides host-driven selective forces, both the live feed and the surrounding rearing environment contribute to shaping the microbiome of farmed gilthead sea-bream larvae, and that a differential establishment of host-associated bacteria takes place during larval development.
dc.description.sponsorshipfor ScienPortuguese Foundation ce and Technology [PTDC/MAR/112792/2009, UID/Multi/04326/2013, UID/BIO/04565/2013]; Programa Operacional Regional de Lisboa [007317]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.3389/fmicb.2017.00204
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10400.1/11313
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFrontiers Media Sa
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSp-Nov.
dc.subjectIntestinal microbiota
dc.subjectEmended description
dc.subjectPseudomonas-Fragi
dc.subjectFish larvae
dc.subjectGen. Nov.
dc.subjectMarine
dc.subjectDiversity
dc.subjectCultivation
dc.subjectAquaculture
dc.titleMolecular Taxonomic Profiling of Bacterial Communities in a Gilthead Seabream (Sparus aurata) Hatchery
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F112792%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIO%2F04565%2F2013/PT
oaire.citation.startPage204
oaire.citation.titleFrontiers in Microbiology
oaire.citation.volume8
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameCalifano
person.familyNameCosta Castanho
person.familyNameCox
person.familyNameda Silva Costa
person.givenNameGianmaria
person.givenNameSara Isabel
person.givenNameCymon
person.givenNameRodrigo
person.identifier115920
person.identifier.ciencia-id5B13-85E1-8549
person.identifier.ciencia-id8015-5A81-BDE2
person.identifier.ciencia-id6B15-9771-1D04
person.identifier.ciencia-id5917-D500-D251
person.identifier.orcid0000-0003-1868-3738
person.identifier.orcid0000-0001-6198-0944
person.identifier.orcid0000-0002-4927-979X
person.identifier.orcid0000-0002-5932-4101
person.identifier.ridD-1303-2012
person.identifier.ridN-7274-2013
person.identifier.scopus-author-id57192871486
person.identifier.scopus-author-id7402112716
person.identifier.scopus-author-id7203063627
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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