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Comparative analysis of isoprostanoid profiles in Chlorella sorokiniana grown under autotrophic and heterotrophic conditions

2025-12Journal article Open access
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dc.contributor.authorConde, Tiago
dc.contributor.authorLopes, Diana
dc.contributor.authorGros, Valérie
dc.contributor.authorReversat, Guillaume
dc.contributor.authorOger, Camille
dc.contributor.authorGalano, Jean-Marie
dc.contributor.authorVigor, Claire
dc.contributor.authorRodrigues, Alexandre M. C.
dc.contributor.authorCoelho, Natacha
dc.contributor.authorCardoso, Helena
dc.contributor.authorDomingues, M. Rosário
dc.contributor.authorDurand, Thierry
dc.date.accessioned2025-12-19T11:29:52Z
dc.date.available2025-12-19T11:29:52Z
dc.date.issued2025-12
dc.description.abstractOxylipins are bioactive lipid mediators derived from polyunsaturated fatty acids (PUFAs), with roles in oxidative stress responses, immunomodulation, and inflammation. While microalgae are recognized as valuable sources of oxylipins, their profiles remain less studied across different species and cultivation conditions. In this study, we characterized the non-enzymatic oxylipin profile of Chlorella sorokiniana grown under autotrophic and heterotrophic conditions to assess the influence of cultivation strategies on their production. A total of 22 isoprostanoids, mainly Phytoprostanes (PhytoP), Phytofurans (PhytoF), Isoprostanes (IsoP), and Neuroprostanes (NeuroP). Autotrophic cultivation resulted in a higher accumulation of isoprostanoids, particularly the alpha-linolenic acid (ALA) derivatives, PhytoP and PhytoF species, likely due to oxidative stress induced by fluctuating light and temperature conditions. In contrast, heterotrophic growth, performed under controlled conditions, yielded lower overall oxylipin levels highlighting the presence of 10(R)-10-F4t-NeuroP which was only present in heterotrophic Chlorella. We observed a correlation between the PUFA composition of Chlorella and its non-enzymatic oxylipin profile. Notably, several oxylipins identified in Chlorella have been associated with antiinflammatory, immunomodulatory, and neuroprotective properties, emphasizing the potential of this micro-alga as a source of high-value bioactive oxylipins. This study paves the way to the utilization of Chlorella as a source of bioactive oxylipins, as well as to develop cultivation strategies to enhance the production of these lipid mediators.eng
dc.identifier.doi10.1016/j.algal.2025.104424
dc.identifier.issn2211-9264
dc.identifier.urihttp://hdl.handle.net/10400.1/27994
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationCentre for Environmental and Marine Studies
dc.relation.ispartofAlgal Research
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAlgae
dc.subjectChlorella
dc.subjectLipidomics
dc.subjectOxylipins
dc.subjectIsoprostanoids
dc.titleComparative analysis of isoprostanoid profiles in Chlorella sorokiniana grown under autotrophic and heterotrophic conditionseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0094%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.citation.titleAlgal Research:Biomass, Biofuels and Bioproducts
oaire.citation.volume92
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCoelho
person.givenNameNatacha
person.identifier.ciencia-idC518-BDB3-E127
person.identifier.orcid0000-0003-4265-5622
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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relation.isAuthorOfPublication.latestForDiscovery43b2d7ec-049a-412b-ad22-c9db64cc572c
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