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Midgut microbiota of the malaria mosquito vector Anopheles gambiae and Interactions with plasmodium falciparum Infection

2012-05Journal article Open access
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dc.contributor.authorBoissiere, Anne
dc.contributor.authorTchioffo, Majoline T.
dc.contributor.authorBachar, Dipankar
dc.contributor.authorAbate, Luc
dc.contributor.authorMarie, Alexandra
dc.contributor.authorNsango, Sandrine E.
dc.contributor.authorShahbazkia, Hamid R.
dc.contributor.authorAwono-Ambene, Parfait H.
dc.contributor.authorLevashina, Elena A.
dc.contributor.authorChristen, Richard
dc.contributor.authorMorlais, Isabelle
dc.date.accessioned2018-12-07T14:53:50Z
dc.date.available2018-12-07T14:53:50Z
dc.date.issued2012-05
dc.description.abstractThe susceptibility of Anopheles mosquitoes to Plasmodium infections relies on complex interactions between the insect vector and the malaria parasite. A number of studies have shown that the mosquito innate immune responses play an important role in controlling the malaria infection and that the strength of parasite clearance is under genetic control, but little is known about the influence of environmental factors on the transmission success. We present here evidence that the composition of the vector gut microbiota is one of the major components that determine the outcome of mosquito infections. A. gambiae mosquitoes collected in natural breeding sites from Cameroon were experimentally challenged with a wild P. falciparum isolate, and their gut bacterial content was submitted for pyrosequencing analysis. The meta-taxogenomic approach revealed a broader richness of the midgut bacterial flora than previously described. Unexpectedly, the majority of bacterial species were found in only a small proportion of mosquitoes, and only 20 genera were shared by 80% of individuals. We show that observed differences in gut bacterial flora of adult mosquitoes is a result of breeding in distinct sites, suggesting that the native aquatic source where larvae were grown determines the composition of the midgut microbiota. Importantly, the abundance of Enterobacteriaceae in the mosquito midgut correlates significantly with the Plasmodium infection status. This striking relationship highlights the role of natural gut environment in parasite transmission. Deciphering microbe-pathogen interactions offers new perspectives to control disease transmission.
dc.description.sponsorshipInstitut de Recherche pour le Developpement (IRD); French Agence Nationale pour la Recherche [ANR-11-BSV7-009-01]; European Community [242095, 223601]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1371/journal.ppat.1002742
dc.identifier.issn1553-7366
dc.identifier.issn1553-7374
dc.identifier.urihttp://hdl.handle.net/10400.1/11712
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Library of Science
dc.relationTowards the establishment of a permanent European Virtual Institute dedicated to Malaria Research (EVIMalaR).
dc.relationPopulation biology and molecular genetics of vectorial capacity in Anopheles gambiae: targeting reproductive behaviour and immunity for transmission-refractory interventions
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMolecular-forms
dc.subjectSporogonic development
dc.subjectBacterial diversity
dc.subjectGut microbiota
dc.subjectIntestinal microbiota
dc.subjectDiptera
dc.subjectCommunities
dc.subjectCulicidae
dc.subjectBurkholderia
dc.subjectEnvironment
dc.titleMidgut microbiota of the malaria mosquito vector Anopheles gambiae and Interactions with plasmodium falciparum Infection
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTowards the establishment of a permanent European Virtual Institute dedicated to Malaria Research (EVIMalaR).
oaire.awardTitlePopulation biology and molecular genetics of vectorial capacity in Anopheles gambiae: targeting reproductive behaviour and immunity for transmission-refractory interventions
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/242095/EU
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/223601/EU
oaire.citation.issue5
oaire.citation.startPagee1002742
oaire.citation.titlePLoS Pathogens
oaire.citation.volume8
oaire.fundingStreamFP7
oaire.fundingStreamFP7
person.familyNameShahbazkia
person.givenNameHamid Reza
person.identifier.ciencia-id5D12-F388-568E
person.identifier.orcid0000-0002-5661-0612
person.identifier.scopus-author-id22735637800
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
project.funder.nameEuropean Commission
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication11ed60f1-defe-4034-9203-633a116cf217
relation.isAuthorOfPublication.latestForDiscovery11ed60f1-defe-4034-9203-633a116cf217
relation.isProjectOfPublicationa7f85f6d-76cf-45ea-a972-49c2d6707f6d
relation.isProjectOfPublication0c6edcb6-c5ce-4b84-95e0-22997315c34f
relation.isProjectOfPublication.latestForDiscovery0c6edcb6-c5ce-4b84-95e0-22997315c34f

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