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Optimizing chlorella vulgaris production and exploring its impact on germination through microalga-N2-fixing bacteria consortia

2025-12Journal article Open access
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dc.contributor.authorSanchez-Zurano, Ana
dc.contributor.authorVilaró-Cos, Silvia
dc.contributor.authorRodrigues Figueiredo, Daniel
dc.contributor.authorMelkonyan, Lusine
dc.contributor.authorFerreira, Alice
dc.contributor.authorAcién, Francisco Gabriel
dc.contributor.authorLafarga, Tomas
dc.contributor.authorGouveia, Luisa
dc.date.accessioned2025-10-27T10:12:41Z
dc.date.available2025-10-27T10:12:41Z
dc.date.issued2025-12
dc.description.abstractMicroalgal biomass is increasingly valued in industrial and agricultural sectors due to its bioactive compounds. However, large-scale production remains costly, mainly due to nitrogen fertilizer expenses. A promising sustainable alternative is co-cultivation with N2-fixing bacteria, capable of supplying biologically available nitrogen. In this study, Chlorella vulgaris was grown in synthetic medium with and without nitrogen, as well as in co-culture with three different N2-fixing bacteria in nitrogen-free medium. Microalgal growth was assessed by dry weight, Fv/Fm ratio, and flow cytometry, which also allowed evaluation of population dynamics and cell viability. Biomass composition (proteins, carbohydrates, lipids, chlorophyll, and carotenoids) was analyzed under all conditions. Co-cultures in nitrogen-free medium showed comparable biomass productivity to nitrogensupplemented controls, although Fv/Fm values indicated physiological stress in some cases. Moreover, the agricultural potential of the resulting biomass and supernatants was evaluated through germination bioassays using lettuce seeds. All cultures tested at 0.2 g⋅L− 1 significantly improved the germination index. Also, applying the culture supernatant (biomass removed) also yielded positive effects, with GI increases exceeding 40 %. These results suggest that co-cultivation with N2-fixing bacteria can support efficient microalgal production while generating biomass and supernatants with biostimulant potential, contributing to sustainable agriculture and circular bioeconomy strategies.eng
dc.description.sponsorshipLisboa-01–0247-FEDER-035234; LISBOA-01–0145-FEDER-022059; 2022.11872.BDANA, project N◦ 21T-2I229, PRODIGIO, 101007006
dc.identifier.doi10.1016/j.jbiotec.2025.09.018
dc.identifier.issn0168-1656
dc.identifier.urihttp://hdl.handle.net/10400.1/27851
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationDevelopment of agricultural bioproducts biofertilizers, biostimulants and biopesticides from piggery wastewater treatment-based microalgae
dc.relationCombination of Constructed Vertical flow Wetlands, Microalgae Photobioreactor and Microbial Fuel Cell (KIT) for wastewater treatment in small pig production farms
dc.relation.ispartofJournal of Biotechnology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMicroalgae
dc.subjectNitrogen-fixing-bacteria
dc.subjectNitrogen
dc.subjectBioestimulants
dc.subjectCytometry
dc.titleOptimizing chlorella vulgaris production and exploring its impact on germination through microalga-N2-fixing bacteria consortiaeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment of agricultural bioproducts biofertilizers, biostimulants and biopesticides from piggery wastewater treatment-based microalgae
oaire.awardTitleCombination of Constructed Vertical flow Wetlands, Microalgae Photobioreactor and Microbial Fuel Cell (KIT) for wastewater treatment in small pig production farms
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F144122%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/DRI%2FIndia%2F0609%2F2020/PT
oaire.citation.endPage253
oaire.citation.startPage244
oaire.citation.titleJournal of Biotechnology
oaire.citation.volume408
oaire.fundingStream3599-PPCDT
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameRodrigues Figueiredo
person.familyNameGouveia
person.givenNameDaniel
person.givenNameLuisa
person.identifierH-4046-2011
person.identifier.ciencia-id181D-2CFA-B65A
person.identifier.ciencia-id581B-924A-84DD
person.identifier.orcid0000-0003-3201-5736
person.identifier.orcid0000-0003-0196-6360
person.identifier.scopus-author-id7004135834
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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relation.isAuthorOfPublicationf559d57b-b659-4d65-bf6c-698bf99e729b
relation.isAuthorOfPublication.latestForDiscoveryf539897e-73bb-4f05-86a9-c30eeb10fbb7
relation.isProjectOfPublicationf20303e7-db30-48d5-a3be-2a502d408046
relation.isProjectOfPublication5b9ae94b-c9c5-4af0-8f47-a25deb141081
relation.isProjectOfPublication.latestForDiscoveryf20303e7-db30-48d5-a3be-2a502d408046

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