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Development of a highly sensitive bacteria detection assay using fluorescent pH-responsive polymeric micelles

2011-03Journal article Restricted access
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dc.contributor.authorMouffouk, Fouzi
dc.contributor.authorRosa da Costa, Ana
dc.contributor.authorMartins, Jorge
dc.contributor.authorZourob, Mohammed
dc.contributor.authorAbu-Salah, Khalid
dc.contributor.authorAlrokayan, Salman A.
dc.date.accessioned2015-09-09T09:16:26Z
dc.date.available2015-09-09T09:16:26Z
dc.date.issued2011-03
dc.description.abstractThe detection and control of bacteria is extremely important in the safety of food products and health systems. The conventional microbiological methods based on culture enrichment techniques and plating procedures are highly sensitive and selective for bacterial detection but are expensive, cumbersome and time-consuming. Here we report the development of a simple and sensitive bioassay to detect Escherichia coli (E. coli) bacteria by using self assembled pH-responsive polymeric micelles that have been bioconjugated to anti-E. coli (capturing agent). Poly(ethylene glycol-b-trimethylsilyl methacrylate), containing silicon moieties that can be cleaved under mildly acidic conditions, was synthesized and selfassembled into micelles, that were loaded with a fluorescent dye (1-methylpyrene). The polymer silicon protecting groups are used as a tool to remotely activate the dye release by means of pH. The high sensitivity of the newly developed bioassay, which is capable of detecting 15 bacteria per milliliter of solution, is due to an amplification effect generated by the optical signal of millions of fluorophores released from a single micelle upon attachment to a bacterium. Fluorescence probing involves the measurements of changes in the emission spectra, through the disappearance of the excimer band, which only occurs when the dye molecules are trapped within the polymeric micelles.por
dc.description.sponsorshipThis work was supported by the research chair on targeting and treatment of cancer with nanoparticles, King Saud University, Saudi Arabia; Centro de Biomedicina Molecular e Estrutural, IBB/CBME, LA, FEDER/POCI 2010, and Fundac¸ ão para a Ciência e Tecnologia, Portugal
dc.identifier.citationBiosensors and Bioelectronics 26 (2011) 3517–3523por
dc.identifier.doi10.1016/j.bios.2011.01.037
dc.identifier.issn0956-5663
dc.identifier.otherAUT: AMC01695;
dc.identifier.urihttp://hdl.handle.net/10400.1/6750
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://ac.els-cdn.com/S0956566311000613/1-s2.0-S0956566311000613-main.pdf?_tid=45c6f7be-532b-11e5-8831-00000aab0f26&acdnat=1441388282_61ed19eb6859b58d76e835494623c5c9por
dc.subjectBiosensorspor
dc.subjectBacteria detectionpor
dc.subjectPoly(ethylene glycol-b-trimethylsilylmethacrylate)por
dc.subjectSelf assembled polymerspor
dc.subjectpH sensitive micellespor
dc.subjectPyrenyl fluorescencepor
dc.titleDevelopment of a highly sensitive bacteria detection assay using fluorescent pH-responsive polymeric micellespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3523por
oaire.citation.issue8por
oaire.citation.startPage3517por
oaire.citation.titleBiosensors and Bioelectronicspor
oaire.citation.volume26por
person.familyNameRosa da Costa
person.givenNameAna M
person.identifier305029
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.orcid0000-0003-0225-9537
person.identifier.ridE-2165-2012
person.identifier.scopus-author-id53986075100
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication0ca8c16f-6881-4662-bafc-cc51c5ce8d88
relation.isAuthorOfPublication.latestForDiscovery0ca8c16f-6881-4662-bafc-cc51c5ce8d88

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