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The gorgonian coral Eunicella labiata hosts a distinct prokaryotic consortium amenable to cultivation

2017-12Journal article Open access
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dc.contributor.authorKeller-Costa, Tina
dc.contributor.authorEriksson, Dominic
dc.contributor.authorGonçalves, Jorge Manuel Santos
dc.contributor.authorGomes, Newton C. M.
dc.contributor.authorLago-Lestón, Asunción
dc.contributor.authorCosta, Rodrigo
dc.date.accessioned2019-11-20T15:07:08Z
dc.date.available2019-11-20T15:07:08Z
dc.date.issued2017-12
dc.description.abstractMicrobial communities inhabiting gorgonian corals are believed to benefit their hosts through nutrient provision and chemical defence; yet much remains to be learned about their phylogenetic uniqueness and cultivability. Here, we determined the prokaryotic community structure and distinctiveness in the gorgonian Eunicella labiata by Illumina sequencing of 16S rRNA genes from gorgonian and seawater metagenomic DNA. Furthermore, we used a 'plate-wash' methodology to compare the phylogenetic diversity of the 'total' gorgonian bacteriome and its 'cultivatable' fraction. With 1016 operational taxonomic units (OTUs), prokaryotic richness was higher in seawater than in E. labiata where 603 OTUs were detected, 68 of which were host-specific. Oceanospirillales and Rhodobacterales predominated in the E. labiata communities. One Oceanospirillales OTU, classified as Endozoicomonas, was particularly dominant, and closest relatives comprised exclusively uncultured clones from other gorgonians. We cultivated a remarkable 62% of the bacterial symbionts inhabiting E. labiata: Ruegeria, Sphingorhabdus, Labrenzia, other unclassified Rhodobacteraceae, Vibrio and Shewanella ranked among the 10 most abundant genera in both the cultivation-independent and dependent samples. In conclusion, the E. labiata microbiome is diverse, distinct from seawater and enriched in (gorgonian)-specific bacterial phylotypes. In contrast to current understanding, many dominant E. labiata symbionts can, indeed, be cultivated.
dc.description.sponsorshipPortuguese Foundation for Science and Technology [EXPL/MAR-EST/1664/2013, SFRH/BPD/34282/2006]
dc.description.sponsorshipGerman Volkswagen (VW) foundation [Az. 81 040-2]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1093/femsec/fix143
dc.identifier.issn0168-6496
dc.identifier.issn1574-6941
dc.identifier.urihttp://hdl.handle.net/10400.1/12903
dc.language.isoeng
dc.peerreviewedyes
dc.publisherOxford Univ Press
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBacterial Communities
dc.subjectMass-Mortality
dc.subjectMicrobial Communities
dc.subjectGenus Endozoicomonas
dc.subjectDiversity
dc.subjectDisease
dc.subjectHealth
dc.subjectReef
dc.subjectSea
dc.subjectMicroorganisms
dc.titleThe gorgonian coral Eunicella labiata hosts a distinct prokaryotic consortium amenable to cultivation
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue12
oaire.citation.startPagefix143
oaire.citation.titleFems Microbiology Ecology
oaire.citation.volume93
person.familyNameGonçalves
person.givenNameJorge Manuel Santos
person.identifier356846
person.identifier.ciencia-id1013-ED6A-65EB
person.identifier.orcid0000-0001-7704-8190
person.identifier.ridG-5206-2011
person.identifier.scopus-author-id7103326116
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublicationdd7829e9-e294-4297-8fb2-adf83c8623cf
relation.isAuthorOfPublication.latestForDiscoverydd7829e9-e294-4297-8fb2-adf83c8623cf

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