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PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challenge

2010-08Journal article Restricted access
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dc.contributor.authorFuentes, J.
dc.contributor.authorBrinca, Lilia
dc.contributor.authorGuerreiro, P. M.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-24T14:34:24Z
dc.date.available2014-10-24T14:34:24Z
dc.date.issued2010-08
dc.description.abstractThe endocrine factors prolactin (PRL) and growth hormone (GH) are believed to have counteracting effects in the adaption of fish to changes in environmental salinity. In order to further investigate this interaction sea bream were challenged with full seawater (SW) or freshwater (FW) for 7 days and the response of pituitary glands cultured in vitro to an osmotic challenge (230, 275 and 320 mOsm/kg) was assessed. In vitro PRL secretion from pituitaries of SW-adapted fish was unaltered in response to an osmotic challenge, while GH secretion increased in the lowest osmolality (230 mOsm/kg). In contrast, both GH and PRL secretion by pituitaries from FW challenged fish was significantly increased (p < 0.01) over that of pituitaries from SW fish at the highest osmolality (320 mOsm/kg). After FW challenge pituitary PRL content and de novo synthesised and released PRL were significantly increased (p < 0.01), while total PRL secretion was not different from SW animals. GH pituitary content decreased in FW animals while total secretion and secretion of de novo synthesised protein were significantly increased (p < 0.01). In addition, after transfer of fish to FW expression of PRL and GH increased 3- and 2-fold, respectively. Despite the increase in PRL expression, no increase in total PRL secretion occurred and although in gills a 2-fold increase in the osmoregulatory marker, Na+/K+-ATPase activity was detected, profound haemodilution and a cumulative mortality of 40% occurred in sea bream placed in FW. Taken together the results suggest that the sea bream pituitary gland fails to respond appropriately to the osmotic challenge caused by low salinity and the physiological response evoked in vivo is not enough to allow this species to withstand and adapt to FW.por
dc.description.sponsorshipThe present work was funded by the Ministry of Science and Technology (FCT), Portugal, project PRAXIS/P/BIA/13174/98 and pluriannual funding to the Centre of Marine Sciences (CCMar). L. Brinca and P.M. Guerreiro were funded by the Ministry of Science (FCT), Portugal through Grants, 4/5.3/PRODEP/2000 and BPD/9464/ 2002, respectively. The authors express their appreciation to Dr. Patricia M. Ingleton for helpful discussion about the study. The constructive critique and helpful comments of two anonymous reviewers’ contributed to improve the manuscript.por
dc.identifier.citationJuan Fuentes, Lilia Brinca, Pedro M. Guerreiro, Deborah M. Power, "PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challenge" in General and Comparative Endocrinology 168 (2010) 95–102.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2010.04.005
dc.identifier.issn0016-6480
dc.identifier.otherAUT: PLBR00503; MG01122; DPO00386
dc.identifier.urihttp://hdl.handle.net/10400.1/5505
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0016648010001528por
dc.subjectGill Na+/K+-ATPasepor
dc.subjectOsmoregulationpor
dc.subjectPituitary glandpor
dc.subjectProlactinpor
dc.subjectGrowth hormonepor
dc.subjectTeleostpor
dc.titlePRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challengepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage102por
oaire.citation.issue1por
oaire.citation.startPage95por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume168por
person.familyNameFuentes
person.familyNameBrinca
person.familyNameGuerreiro da Costa Guerreiro
person.familyNamePower
person.givenNameJuan
person.givenNameLilia
person.givenNamePedro Miguel
person.givenNameDeborah Mary
person.identifier392998
person.identifierA-2539-2009
person.identifier.ciencia-id421B-E196-2C33
person.identifier.ciencia-id5C13-965D-3148
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-0430-8734
person.identifier.orcid0000-0001-9135-8646
person.identifier.orcid0000-0001-5371-7919
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7201832526
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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