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Silencing of the FRO1 gene and its effects on iron partition in Nicotiana benthamiana

2017-05Journal article Restricted
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dc.contributor.authorGama, Florinda
dc.contributor.authorSaavedra, Teresa
dc.contributor.authorDandlen, Susana
dc.contributor.authorde Varennes, Amarilis
dc.contributor.authorCorreia, Pedro José
dc.contributor.authorPestana, Maribela
dc.contributor.authorNolasco, Gustavo
dc.date.accessioned2019-11-20T15:07:39Z
dc.date.available2019-11-20T15:07:39Z
dc.date.issued2017-05
dc.description.abstractTo evaluate the dynamic role of the ferric-chelate reductase enzyme (FCR) and to identify possible pathways of regulation of its activity in different plant organs an investigation was conducted by virus induced gene silencing (VIGS) using tobacco rattle virus (TRV) to silence the ferric reductase oxidase gene (FROI) that encodes the FCR enzyme. Half of Nicotiana benthamiana plants received the VIGS vector and the rest remained as control. Four treatments were imposed: two levels of Fe in the nutrient solution (0 or 2.5 mu M of Fe), each one with silenced or non-silenced (VIGS-0; VIGS-2.5) plants. Plants grown without iron (0; VIGS-0) developed typical symptoms of iron deficiency in the youngest leaves. To prove that FROI silencing had occurred, resupply of Fe (R) was done by adding 2.5 mu M of Fe to the nutrient solution in a subset of chlorotic plants (0-R; VIGS-R). Twelve days after resupply, 0-R plants had recovered from Fe deficiency while plants containing the VIGS vector (VIGS-R) remained chlorotic and both FRO1 gene expression and FCR activity were considerably reduced, consequently preventing Fe uptake. With the VIGS technique we were able to silence the FRO1 gene in N. benthamiana and point out its importance in chlorophyll synthesis and Fe partition. (C) 2017 Elsevier Masson SAS. All rights reserved.
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.plaphy.2017.03.004
dc.identifier.issn0981-9428
dc.identifier.urihttp://hdl.handle.net/10400.1/13146
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier France-Editions Scientifiques Medicales Elsevier
dc.relationTransport of iron in plants: novel approaches to an old problem. Improved iron mobility and development of a new fertilizer based on grass-clipping extracts.
dc.relationIRON TRAFFICKING AND SIGNALLING IN STRAWBERRY PLANTS
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectChelate reductase-activity
dc.subjectStrawberry plants
dc.subjectFerric reductase
dc.subjectDeficiency
dc.subjectRoots
dc.subjectArabidopsis
dc.subjectVirus
dc.subjectExpression
dc.subjectTomato
dc.subjectTransporter
dc.titleSilencing of the FRO1 gene and its effects on iron partition in Nicotiana benthamiana
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTransport of iron in plants: novel approaches to an old problem. Improved iron mobility and development of a new fertilizer based on grass-clipping extracts.
oaire.awardTitleIRON TRAFFICKING AND SIGNALLING IN STRAWBERRY PLANTS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-PRO%2F3861%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F89521%2F2012/PT
oaire.citation.endPage118
oaire.citation.startPage111
oaire.citation.titlePlant Physiology and Biochemistry
oaire.citation.volume114
oaire.fundingStream3599-PPCDT
person.familyNameGama
person.familyNameSaavedra
person.familyNameDandlen
person.familyNameCorreia
person.familyNamePestana
person.familyNameNolasco
person.givenNameFlorinda
person.givenNameTeresa
person.givenNameSusana Anahi
person.givenNamePedro José
person.givenNameMaribela
person.givenNameGustavo
person.identifier74793
person.identifier.ciencia-idB61D-C9B5-8AE2
person.identifier.ciencia-id3F1F-53D6-1014
person.identifier.ciencia-id6613-CD28-84BE
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person.identifier.ciencia-id791E-9EC5-55C9
person.identifier.orcid0000-0001-6948-0055
person.identifier.orcid0000-0002-2125-6342
person.identifier.orcid0000-0003-4927-1189
person.identifier.orcid0000-0002-4319-1682
person.identifier.orcid0000-0002-7025-6023
person.identifier.ridF-5741-2010
person.identifier.scopus-author-id35770492700
person.identifier.scopus-author-id36467141800
person.identifier.scopus-author-id7006210686
person.identifier.scopus-author-id7005146039
person.identifier.scopus-author-id6602625528
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
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