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Molecular and functional characterization of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase from Dunaliella salina

2009Journal article Restricted access
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dc.contributor.authorRamos, Ana
dc.contributor.authorMarques, A. R.
dc.contributor.authorRodrigues, M.
dc.contributor.authorHenriques, Nuno
dc.contributor.authorBaumgartner, Alexandra
dc.contributor.authorCastilho, Rita
dc.contributor.authorBrenig, Bertram
dc.contributor.authorVarela, J.
dc.date.accessioned2014-05-20T12:25:39Z
dc.date.available2014-05-20T12:25:39Z
dc.date.issued2009
dc.date.updated2014-05-16T11:03:44Z
dc.description.abstractIn green algae,the final step of the plastidial methylerythritol phosphate (MEP) pathway is catalyzed by 4-hydroxy-3-methylbut-2-enyldiphosphate reductase(HDR; EC: 1.17.1.2),an enzyme proposed to play a keyrole in the regulation of isoprenoid biosynthesis. Here we report the isolation and functional characterization of a 1959- bp Dunaliella salina HDR (DsHDR) cDNA encoding a deduced polypeptide of 474 amino acid residues. Phylogenetic analysis implied a cyanobacterial origin for plant and algal HDR genes. Steady-state DsHDR transcript levels were higher in D. salina cells submitted to nutritional depletion, high salt and/or high light, suggesting that DsHDR may respond to the same environmental cues as genes involved in carotenoid biosynthesis.por
dc.description.sponsorshipOVERCAROTEN POCTI/MAR/15237/99
dc.description.sponsorshipINTERREG159-SAL-AtlanticSaltPonds
dc.identifier.citationRamos, A. A.; Marques, A. R.; Rodrigues, M.; Henriques, N.; Baumgartner, A.; Castilho, R.; Brenig, B.; Varela, J. C. Molecular and functional characterization of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase from Dunaliella salina, Journal of Plant Physiology, 166, 9, 968-977, 2009.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.jplph.2008.11.008
dc.identifier.issn0176-1617
dc.identifier.otherAUT: JVA01305; RCA00270;
dc.identifier.urihttp://hdl.handle.net/10400.1/4016
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationMOLECULAR BASIS OF ISOPRENOID BIOSYNTHESIS IN DUNALIELLA SALINA
dc.relationREGULATION OF THE CAROTENOID BIOSYNTHETIC PATHWAY IN THE HALOTOLERANT MICROALGA DUNALIELLA SALINA
dc.subjectCarotenogenesispor
dc.subjectDunaliella salinapor
dc.subject4-Hydroxy-3-methylbut-2-enyldiphosphate reductasepor
dc.subjectIsoprenoid biosynthesispor
dc.subjectMethylerythritol phosphate pathwaypor
dc.titleMolecular and functional characterization of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase from Dunaliella salinapor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMOLECULAR BASIS OF ISOPRENOID BIOSYNTHESIS IN DUNALIELLA SALINA
oaire.awardTitleREGULATION OF THE CAROTENOID BIOSYNTHETIC PATHWAY IN THE HALOTOLERANT MICROALGA DUNALIELLA SALINA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F13937%2F2003/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F4839%2F2001/PT
oaire.citation.endPage977por
oaire.citation.issue9por
oaire.citation.startPage968por
oaire.citation.titleJournal of Plant Physiologypor
oaire.citation.volume166por
person.familyNameRamos
person.familyNameCastilho
person.familyNameVarela
person.givenNameAna
person.givenNameRita
person.givenNameJoão
person.identifier452212
person.identifier.ciencia-id761B-F84D-0CF5
person.identifier.ciencia-id0513-0407-A6C1
person.identifier.orcid0000-0003-1099-4005
person.identifier.orcid0000-0003-0727-3688
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridM-3815-2013
person.identifier.ridB-6185-2008
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id56271196600
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication42936018-1328-4fd1-8adb-4d37441727ab
relation.isAuthorOfPublication051f68ec-18e0-4008-b06d-bf4b996fa098
relation.isAuthorOfPublicationd339a9e7-bc32-49c4-ba48-a17343d40092
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relation.isProjectOfPublication.latestForDiscovery87f3d167-a603-4907-9364-be92fab44fda

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