Repository logo
 
Publication

Comparative analysis of zebrafish bone morphogenetic proteins 2, 4 and 16: molecular and evolutionary perspectives

2016-02Journal article Open access
Simple item page
dc.contributor.authorMarques, Cátia L.
dc.contributor.authorFernandez, Ignacio
dc.contributor.authorViegas, Michael
dc.contributor.authorCox, C. J.
dc.contributor.authorMartel, Paulo
dc.contributor.authorRosa, Joana
dc.contributor.authorCancela, Leonor
dc.contributor.authorLaizé, Vincent
dc.date.accessioned2017-04-07T15:57:13Z
dc.date.available2017-04-07T15:57:13Z
dc.date.issued2016-02
dc.description.abstractBMP2, BMP4 and BMP16 form a subfamily of bone morphogenetic proteins acting as pleiotropic growth factors during development and as bone inducers during osteogenesis. BMP16 is the most recent member of this subfamily and basic data regarding protein structure and function, and spatio-temporal gene expression is still scarce. In this work, insights on BMP16 were provided through the comparative analysis of structural and functional data for zebrafish BMP2a, BMP2b, BMP4 and BMP16 genes and proteins, determined from three-dimensional models, patterns of gene expression during development and in adult tissues, regulation by retinoic acid and capacity to activate BMP-signaling pathway. Structures of Bmp2a, Bmp2b, Bmp4 and Bmp16 were found to be remarkably similar; with residues involved in receptor binding being highly conserved. All proteins could activate the BMP-signaling pathway, suggesting that they share a common function. On the contrary, stage-and tissue-specific expression of bmp2, bmp4 and bmp16 suggested the genes might be differentially regulated (e.g. different transcription factors, enhancers and/or regulatory modules) but also that they are involved in distinct physiological processes, although with the same function. Retinoic acid, a morphogen known to interact with BMP-signaling during bone formation, was shown to downregulate the expression of bmp2, bmp4 and bmp16, although to different extents. Taxonomic and phylogenetic analyses indicated that bmp16 diverged before bmp2 and bmp4, is not restricted to teleost fish lineage as previously reported, and that it probably arose from a whole genomic duplication event that occurred early in vertebrate evolution and disappeared in various tetrapod lineages through independent events.
dc.identifier.doi10.1007/s00018-015-2024-x
dc.identifier.issn1420-682X
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/9644
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Verlag
dc.relation.isbasedonWOS:000369535300010
dc.subjectBMP-signaling
dc.subjectBMP2/4/16 subfamily
dc.subjectBone morphogenetic proteins
dc.subjectEvolution
dc.subjectGene expression
dc.subjectRetinoic acid
dc.titleComparative analysis of zebrafish bone morphogenetic proteins 2, 4 and 16: molecular and evolutionary perspectives
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage857
oaire.citation.issue4
oaire.citation.startPage841
oaire.citation.titleCellular and Molecular Life Sciences
oaire.citation.volume73
person.familyNameViegas
person.familyNameCox
person.familyNameMartel
person.familyNameRosa
person.familyNameCancela
person.familyNameLaizé
person.givenNameMichael
person.givenNameCymon
person.givenNamePaulo
person.givenNameJoana
person.givenNameM. Leonor
person.givenNameVincent
person.identifier1955829
person.identifier.ciencia-idAC1C-93D9-7A83
person.identifier.ciencia-id6B15-9771-1D04
person.identifier.ciencia-idAD16-8C2A-26CE
person.identifier.ciencia-id0B18-8AEC-875C
person.identifier.orcid0000-0002-0152-213X
person.identifier.orcid0000-0002-4927-979X
person.identifier.orcid0000-0001-8237-1267
person.identifier.orcid0000-0001-7947-2681
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridD-1303-2012
person.identifier.ridK-4704-2013
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id36505309900
person.identifier.scopus-author-id7402112716
person.identifier.scopus-author-id7101625192
person.identifier.scopus-author-id36505067900
person.identifier.scopus-author-id6602982778
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublicationb6a944d4-49ac-4ead-a8ae-59ef473045d5
relation.isAuthorOfPublication82c3689c-60b6-440d-9d7b-49e6dbd6861b
relation.isAuthorOfPublicatione70c3a99-3cde-4b3d-8ca2-4ac252906ae3
relation.isAuthorOfPublication181f1017-5086-40dc-9edc-a289aaa2d263
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublication.latestForDiscoverye70c3a99-3cde-4b3d-8ca2-4ac252906ae3

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
handle9644.pdf
Size:
2.34 MB
Format:
Adobe Portable Document Format
Download

Collections

FCB2-Artigos (em revistas ou actas indexadas)
CCM2-Artigos (em revistas ou actas indexadas)