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Mid-infrared spectroscopic screening of metabolic alterations in stress-exposed gilthead seabream (Sparus aurata)

2020Journal article Open access
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dc.contributor.authorRaposo de Magalhães, Cláudia
dc.contributor.authorCarrilho, Raquel
dc.contributor.authorSchrama, Denise
dc.contributor.authorCerqueira, Marco
dc.contributor.authorRosa Da Costa, Ana
dc.contributor.authorRodrigues, Pedro
dc.date.accessioned2020-11-05T21:22:57Z
dc.date.available2020-11-05T21:22:57Z
dc.date.issued2020
dc.description.abstractStress triggers a battery of physiological responses in fish, including the activation of metabolic pathways involved in energy production, which helps the animal to cope with the adverse situation. Prolonged exposure to stressful farming conditions may induce adverse effects at the whole-animal level, impairing welfare. Fourier transform infrared (FTIR) spectroscopy is a rapid biochemical fingerprinting technique, that, combined with chemometrics, was applied to disclose the metabolic alterations in the fish liver as a result of exposure to standard stressful practices in aquaculture. Gilthead seabream (Sparus aurata) adults exposed to different stressors were used as model species. Spectra were preprocessed before multivariate statistical analysis. Principal components analysis (PCA) was used for pattern recognition and identification of the most discriminatory wavenumbers. Key spectral features were selected and used for classification using the k-nearest neighbour (KNN) algorithm to evaluate whether the spectral changes allowed for the reliable discrimination between experimental groups. PCA loadings suggested that major variations in the hepatic infrared spectra responsible for the discrimination between the experimental groups were due to differences in the intensity of absorption bands associated with proteins, lipids and carbohydrates. This broad-range technique can thus be useful in an exploratory approach before any targeted analysis.pt_PT
dc.description.sponsorshipFCT: SFRH/BD/138884/2018/ SFRH/BD/136319/2018/ UIDB/04326/2020/ 16-02-05-FMP-12pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41598-020-73338-zpt_PT
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.1/14809
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Researchpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectTransform infrared-spectroscopypt_PT
dc.subjectRainbow-trout liverpt_PT
dc.subjectSalmon salmo-salarpt_PT
dc.subjectFt-irpt_PT
dc.subjectCancer classificationpt_PT
dc.subjectGene selectionpt_PT
dc.subjectExpressionpt_PT
dc.subjectSpoilagept_PT
dc.titleMid-infrared spectroscopic screening of metabolic alterations in stress-exposed gilthead seabream (Sparus aurata)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.startPage16343pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume10pt_PT
person.familyNameRaposo de Magalhães
person.familyNameVaz Carrilho
person.familyNameSchrama
person.familyNameCerqueira
person.familyNameRosa da Costa
person.familyNameRodrigues
person.givenNameCláudia
person.givenNameRaquel
person.givenNameDenise
person.givenNameMarco
person.givenNameAna M
person.givenNamePedro
person.identifier159154
person.identifier305029
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person.identifier.orcid0000-0001-7237-5053
person.identifier.orcid0000-0003-0225-9537
person.identifier.orcid0000-0002-9668-1204
person.identifier.ridH-6511-2016
person.identifier.ridE-2165-2012
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id55755583400
person.identifier.scopus-author-id53986075100
person.identifier.scopus-author-id55107531000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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