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Dietary protein complexity modulates growth, protein utilisation and the expression of protein digestion-related genes in Senegalese sole larvae

dc.contributor.authorCanada, Paula
dc.contributor.authorConceicao, Luis E. C.
dc.contributor.authorMira, Sara
dc.contributor.authorTeodósio, Rita
dc.contributor.authorFernandes, Jorge M. O.
dc.contributor.authorBarrios, Carmen
dc.contributor.authorMillan, Francisco
dc.contributor.authorPedroche, Justo
dc.contributor.authorValente, Luisa M. P.
dc.contributor.authorEngrola, Sofia
dc.date.accessioned2019-11-20T15:07:20Z
dc.date.available2019-11-20T15:07:20Z
dc.date.issued2017-10
dc.description.abstractGiven its complex metamorphosis and digestive system ontogeny, Senegalese sole larvae capacity to digest and utilize dietary protein is likely to change throughout development. In the present study, we hypothesized that the manipulation of dietary protein complexity may affect Senegalese sole larvae capacity to digest, absorb and retain protein during metamorphosis, as well as the mRNA expression of genes encoding for the precursors of proteolytic enzymes of the digestive tract and the enterocyte peptide transporter PepT1, which may have further impact on somatic growth. Three diets were formulated using approximately the same practical ingredients, except for the main protein source. The Intact diet protein content was mostly based on intact plant protein where the target peptide molecular weight (MW) would be > 70 kDa. The PartH diet protein fraction was mostly based on a protein hydrolysate with a high content of 5-70 kDa peptides. The HighH diet protein fraction was mostly based on a protein hydrolysate with a high content of 5 kDa peptides. A growth trial was performed with larvae reared at 19 degrees C under a co-feeding regime from mouth opening. The transcription of pga, tryp1c, ialp, ampn and pepT1 (encoding respectively for PepsinogenA, Trypsinogen1C, Intestinal alkaline phosphatase, Aminopeptidase N and for the enterocyte peptide transporter 1) was quantified by qPCR, during the metamorphosis climax (16 DAH) and after the metamorphosis was completed (28 DAH). An in vivo method of controlled tube-feeding was used to assess the effect on the larvae capacity to utilize polypeptides with different MW (1.0 and 7.2 kDa) representing a typical peptide MW of each of the hydrolysates included in the diets. The PartH diet stimulated growth in metamorphosing larvae (16 DAH), whereas the Intact diet stimulated growth after 36 DAH. The Intact diet stimulated the larvae absorption capacity for 1.0 kDa peptides at 16 DAH, which may have contributed for enhanced growth in later stages. The PartH diet stimulated the transcription of tryp1c and pept1 at 28 DAH, which seemed to reflect on increased post-larvae capacity to retain dietary 7.2 kDa polypeptides. That may indicate a possible strategy to optimize the digestion and utilisation of the PartH dietary protein, though it did not reflect into increased growth. The Intact diet promoted the transcription of pepsinogenA, which may reflect a reduced gastrointestinal transit time, which could have enhanced the dietary nutrients assimilation, ultimately improving growth. The present results suggest that, whereas pre-metamorphic sole larvae utilize better dietary protein with a moderate degree of hydrolysis, post-metamorphic sole make a greater use of intact protein.
dc.description.sponsorshipProject EPISOLE (FCT) [PTDC/MAR/110547/2009]
dc.description.sponsorshipFCT (Portugal) [CCMAR/Multi/04326/2013 (Portugal)]
dc.description.sponsorshipMICALA - I & DT Co-Promocao I & DT Co-Promocao (Portugal) - POAlgarve 21 [13380]
dc.description.sponsorshipMICALA - I & DT Co-Promocao I & DT Co-Promocao (Portugal) - QREN
dc.description.sponsorshipMICALA - I & DT Co-Promocao I & DT Co-Promocao (Portugal) - European Union
dc.description.sponsorshipFCT [SFRH/BD/82149/2011]
dc.description.sponsorshipFCT investigator grant - European Social Fund [IF/00482/2014/CP1217/CT0005]
dc.description.sponsorshipOperational Programme Human Potential
dc.description.sponsorshipFoundation for Science and Technology of Portugal (FCT)
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.aquaculture.2017.05.028
dc.identifier.issn0044-8486
dc.identifier.issn1873-5622
dc.identifier.urihttp://hdl.handle.net/10400.1/12987
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Science Bv
dc.relationEpigenetic regulation of development and growth in Senegalese sole (Solea senegalensis)- EPISOLE
dc.relationIMPROVING GROWTH POTENTIAL IN SENEGALESE SOLE THROUGH DIETARY PROTEIN: AN INTEGRATED APPROACH USING MUSCLE CELLULARITY, TRACER STUDIES AND GENE EXPRESSION
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBass dicentrarchus-labrax
dc.subjectHalibut hippoglossus-hippoglossus
dc.subjectDiplodus-sargus larvae
dc.subjectSeabream sparus-aurata
dc.subjectAmino-acid supplementation
dc.subjectCod gadus-morhua
dc.subjectFed rotifers
dc.subjectFish larvae
dc.subjectInert diet
dc.subjectEnzymes
dc.titleDietary protein complexity modulates growth, protein utilisation and the expression of protein digestion-related genes in Senegalese sole larvae
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEpigenetic regulation of development and growth in Senegalese sole (Solea senegalensis)- EPISOLE
oaire.awardTitleIMPROVING GROWTH POTENTIAL IN SENEGALESE SOLE THROUGH DIETARY PROTEIN: AN INTEGRATED APPROACH USING MUSCLE CELLULARITY, TRACER STUDIES AND GENE EXPRESSION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FMAR%2F110547%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F82149%2F2011/PT
oaire.citation.endPage284
oaire.citation.startPage273
oaire.citation.titleAquaculture
oaire.citation.volume479
oaire.fundingStream5876-PPCDTI
person.familyNameMorgado Canada
person.familyNameMira
person.familyNameTeodósio
person.familyNameEngrola
person.givenNamePaula Alexandra
person.givenNameSara
person.givenNameRita
person.givenNameSofia
person.identifier237846
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person.identifier.orcid0000-0001-8346-4449
person.identifier.orcid0000-0002-8648-1241
person.identifier.orcid0000-0002-5244-5541
person.identifier.ridA-2485-2012
person.identifier.scopus-author-id6603417249
person.identifier.scopus-author-id35867417300
person.identifier.scopus-author-id8669620600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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