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Molecular cloning of superoxide dismutase (Cu/Zn-SOD) from aquatic molluscs

2004Journal article Restricted access
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dc.contributor.authorGeret, F.
dc.contributor.authorManduzio, H.
dc.contributor.authorCompany, Rui
dc.contributor.authorLeboulenger, F.
dc.contributor.authorBebianno, Maria João
dc.contributor.authorDanger, J. M.
dc.date.accessioned2020-05-11T13:08:40Z
dc.date.available2020-05-11T13:08:40Z
dc.date.issued2004
dc.description.abstractThe potential of the first line of the active oxygen-scavenging system, partial cDNA encoding Cu/Zn superoxide dismutase (SOD) was isolated in three aquatic mollusc species: Ruditapes decussatus (marine clam), Dreissena polymorpha (continental water mussel) and Bathymodiolus azoricus (hydrothermal vent mussel). These SOD cDNA fragments were amplified by PCR with degenerate oligonucleotide primers derived from the amino acid sequence conserved in the Cu/Zn-SOD from several other organisms. A partial cDNA of CuZn-SOD was obtained for R. decussates (510 bp), D. polymorpha (510 bp) and B. azoricus (195 bp). The deduced amino acid sequence showed high similarity among the three mollusc species (57-63%) and among other species (50-65%). The residues involved in coordinating copper (His-47, 49, 64, 121) and zinc (His-64, 72, 81 and Asp-84) were well conserved among the three Cu/Zn-SOD sequences.pt_PT
dc.description.sponsorshipFCT (Fundação para Ciência e a Tecnologia) of the Ministry of Science and Technology of Portugalpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.marenvres.2004.03.052pt_PT
dc.identifier.issn0141-1136
dc.identifier.urihttp://hdl.handle.net/10400.1/13870
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAmino acid sequencept_PT
dc.subjectAnimalspt_PT
dc.subjectBase sequencept_PT
dc.subjectBivalviapt_PT
dc.subjectConserved sequencept_PT
dc.subjectCopperpt_PT
dc.subjectDNA primerspt_PT
dc.subjectComplementary DNApt_PT
dc.subjectEnzyme inductionpt_PT
dc.subjectMolecular sequence datapt_PT
dc.subjectSequence Alignmentpt_PT
dc.subjectDNA sequence analysispt_PT
dc.subjectSequence homologypt_PT
dc.subjectSuperoxide dismutasept_PT
dc.subjectSuperoxide dismutasept_PT
dc.subjectcDNApt_PT
dc.subjectMolluscspt_PT
dc.subjectRuditapes decussatuspt_PT
dc.subjectBathymodiolus azoricuspt_PT
dc.subjectDreissena polymorphapt_PT
dc.titleMolecular cloning of superoxide dismutase (Cu/Zn-SOD) from aquatic molluscspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage623pt_PT
oaire.citation.issue2-5pt_PT
oaire.citation.startPage619pt_PT
oaire.citation.titleMarine Environmental Researchpt_PT
oaire.citation.volume58pt_PT
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32

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