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INsPECT, an open-source and versatile software for automated quantification of (Leishmania) intracellular parasites

2014-05Journal article Open access
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dc.contributor.authorYazdanparast, Ehsan
dc.contributor.authorDos Anjos, António
dc.contributor.authorGarcia, Deborah
dc.contributor.authorLoeuillet, Corinne
dc.contributor.authorShahbazkia, Hamid R.
dc.contributor.authorVergnes, Baptiste
dc.date.accessioned2018-12-07T14:53:49Z
dc.date.available2018-12-07T14:53:49Z
dc.date.issued2014-05
dc.description.abstractIntracellular protozoan parasites are causative agents of infectious diseases that constitute major health problems for developing countries. Leishmania sp., Trypanosoma cruzi or Toxoplasma gondii are all obligate intracellular protozoan parasites that reside and multiply within the host cells of mammals, including humans. Following up intracellular parasite proliferation is therefore an essential and a quotidian task for many laboratories working on primary screening of new natural and synthetic drugs, analyzing drug susceptibility or comparing virulence properties of natural and genetically modified strains. Nevertheless, laborious manual microscopic counting of intracellular parasites is still the most commonly used approach. Here, we present INsPECT (Intracellular ParasitE CounTer), an open-source and platform independent software dedicated to automate infection level measurement based on fluorescent DNA staining. It offers the possibility to choose between different types of analyses (fluorescent DNA acquisitions only or in combination with phase contrast image set to further separate intra-from extracellular parasites), and software running modes (automatic or custom). A proof-of-concept study with intracellular Leishmania infantum parasites stained with DAPI (49,6-diamidino-2-phenylindole) confirms a good correspondence between digital results and the "gold standard" microscopic counting method with Giemsa. Interestingly, this software is versatile enough to accurately detect intracellular T. gondii parasites on images acquired with High Content Screening (HCS) systems. In conclusion, INsPECT software is proposed as a new fast and simple alternative to the classical intracellular Leishmania quantification methods and can be adapted for mid to large-scale drug screening against different intracellular parasites.
dc.description.sponsorshipIRD (Institut de Recherche pour le Development); University of Algarve (Faro, Portugal); "Infectiopole Sud" foundation
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1371/journal.pntd.0002850
dc.identifier.issn1935-2735
dc.identifier.urihttp://hdl.handle.net/10400.1/11704
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Library of Science
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectIn-Vitro
dc.subjectFluorescent protein
dc.subjectAmastigote assay
dc.subjectDrug-resistance
dc.subjectDonovani
dc.subjectMacrophages
dc.subjectAlgorithm
dc.subjectDiscovery
dc.subjectInvitro
dc.titleINsPECT, an open-source and versatile software for automated quantification of (Leishmania) intracellular parasites
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue5
oaire.citation.startPagee2850
oaire.citation.titlePLoS Neglected Tropical Diseases
oaire.citation.volume8
person.familyNameAnjos
person.familyNameShahbazkia
person.givenNameAntónio
person.givenNameHamid Reza
person.identifier.ciencia-id2D11-C8C8-D8EA
person.identifier.ciencia-id5D12-F388-568E
person.identifier.orcid0000-0001-6521-8930
person.identifier.orcid0000-0002-5661-0612
person.identifier.ridM-7604-2013
person.identifier.scopus-author-id25638237700
person.identifier.scopus-author-id22735637800
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublicationc525cee7-e184-4154-ab9a-23270970ca86
relation.isAuthorOfPublication11ed60f1-defe-4034-9203-633a116cf217
relation.isAuthorOfPublication.latestForDiscoveryc525cee7-e184-4154-ab9a-23270970ca86

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