AFLP, ISSR and RAPD markers reveal high levels of genetic diversity among Lupinus spp.

Talhinhas, P.; Neves-Martins, J.; Leitão, J. M.

http://hdl.handle.net/10400.1/6532

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Authors

Talhinhas, P.

Neves-Martins, J.

Leitão, J. M.

Abstract(s)

The Lupinus genus includes a number of important crop species. The use of defined nucleotide sequences for the analysis of genetic diversity among these species has revealed modest levels of diversity. The aim of this study was to access AFLP, ISSR and RAPD markers to evaluate the genetic diversity among L. albus, L. angustifolius, L. cosentinii, L. hispanicus, L. luteus, L. mutabilis, L. pilosus and L. polyphyllus. Unexpectedly, low levels of genetic similarity were found (ranging from 0.205 to 0.432), regardless of the type of molecular marker used. Nevertheless, these techniques consistently showed a greater genetic similarity between L. pilosus and L. cosentinii, L. mutabilis and L. polyphyllus and among L. luteus, L. hispanicus and L. angustifolius, clearly separating the Old World from the New World species. Such low genetic similarity among Lupinus spp. is most unlikely to be due to differences in coding sequences but could be the result of a long diverging process concerning non-coding regions, which would represent a very important proportion of these genomes.