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Transcriptomics reveal an integrative role for maternal thyroid hormones during zebrafish embryogenesis

2017-11Journal article Open access
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dc.contributor.authorSilva, Nadia
dc.contributor.authorLouro, Bruno
dc.contributor.authorTrindade, Marlene
dc.contributor.authorPower, Deborah M.
dc.contributor.authorCampinho, Marco A.
dc.date.accessioned2018-12-07T14:57:54Z
dc.date.available2018-12-07T14:57:54Z
dc.date.issued2017-11
dc.description.abstractThyroid hormones (THs) are essential for embryonic brain development but the genetic mechanisms involved in the action of maternal THs (MTHs) are still largely unknown. As the basis for understanding the underlying genetic mechanisms of MTHs regulation we used an established zebrafish monocarboxylic acid transporter 8 (MCT8) knock-down model and characterised the transcriptome in 25hpf zebrafish embryos. Subsequent mapping of differentially expressed genes using Reactome pathway analysis together with in situ expression analysis and immunohistochemistry revealed the genetic networks and cells under MTHs regulation during zebrafish embryogenesis. We found 4,343 differentially expressed genes and the Reactome pathway analysis revealed that TH is involved in 1681 of these pathways. MTHs regulated the expression of core developmental pathways, such as NOTCH and WNT in a cell specific context. The cellular distribution of neural MTH-target genes demonstrated their cell specific action on neural stem cells and differentiated neuron classes. Taken together our data show that MTHs have a role in zebrafish neurogenesis and suggest they may be involved in cross talk between key pathways in neural development. Given that the observed MCT8 zebrafish knockdown phenotype resembles the symptoms in human patients with Allan-Herndon-Dudley syndrome our data open a window into understanding the genetics of this human congenital condition.
dc.description.sponsorshipPortuguese Fundacao para Ciencia e Tecnologia (FCT) [PTDC/EXPL/MARBIO/0430/2013]; CCMAR FCT Plurianual financing [UID/Multi/04326/2013]; FCT [SFRH/BD/111226/2015, SFRH/BD/108842/2015, SFRH/BPD/89889/2012]; FCT-IF Starting Grant [IF/01274/2014]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1038/s41598-017-16951-9
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10400.1/11757
dc.language.isoeng
dc.peerreviewedyes
dc.publisherNature Publishing Group
dc.relationTRANSCRIPTOMIC RESPONSES TO THERMAL AND OSMOREGULATORY CHALLENGES OF ANTARCTIC NOTOTHENIOID FISH: AN INSIGHT INTO RESPONSES TO GLOBAL CHANGE AND GENOME EVOLUTION
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMonocarboxylate transporter 8
dc.subjectReceptor Alpha-1
dc.subjectGene-expression
dc.subjectBrain-development
dc.subjectNervous-system
dc.subjectMct8
dc.subjectMutations
dc.subjectCells
dc.subjectProtein
dc.subjectHypomyelination
dc.titleTranscriptomics reveal an integrative role for maternal thyroid hormones during zebrafish embryogenesis
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTRANSCRIPTOMIC RESPONSES TO THERMAL AND OSMOREGULATORY CHALLENGES OF ANTARCTIC NOTOTHENIOID FISH: AN INSIGHT INTO RESPONSES TO GLOBAL CHANGE AND GENOME EVOLUTION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F89889%2F2012/PT
oaire.citation.startPage16657
oaire.citation.titleScientific Reports
oaire.citation.volume7
person.familyNameSilva
person.familyNameLouro
person.familyNamePower
person.familyNameCampinho
person.givenNameNádia
person.givenNameBruno
person.givenNameDeborah Mary
person.givenNameMarco António
person.identifier.ciencia-id5D1F-6F3A-A1C2
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id0E18-2560-6EC1
person.identifier.orcid0000-0001-9881-4421
person.identifier.orcid0000-0001-8164-581X
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-5238-0506
person.identifier.ridD-8833-2013
person.identifier.scopus-author-id10244362100
person.identifier.scopus-author-id16028940200
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id8938999600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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