Repository logo
 
Publication

Isolation of a novel aquaglyceroporin from a marine teleost (Sparus auratus) Function and tissue distribution

2004Journal article Open access
Simple item page
dc.contributor.authorSantos, C. R. A.
dc.contributor.authorEstêvão, Dulce
dc.contributor.authorFuentes, J.
dc.contributor.authorCardoso, João CR
dc.contributor.authorFabra, Mercedes
dc.contributor.authorPassos, A. L.
dc.contributor.authorDetmers, F. J.
dc.contributor.authorDeen, P. M. T.
dc.contributor.authorCerda, J.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-24T10:39:05Z
dc.date.available2014-10-24T10:39:05Z
dc.date.issued2004
dc.description.abstractThe aquaporins (formerly called the major intrinsic protein family) are transmembrane channel proteins. The family includes the CHIP group, which are functionally characterised as water channels and the GLP group, which are specialised for glycerol transport. The present study reports the identification and characterisation of a novel GLP family member in a teleost fish, the sea bream Sparus auratus. A sea bream aquaporin (sbAQP) cDNA of 1047·bp and encoding a protein of 298·amino acids was isolated from a kidney cDNA library. Functional characterization of the sbAQP using a Xenopus oocyte assay revealed that the isolated cDNA stimulated osmotic water permeability in a mercury-sensitive manner and also stimulated urea and glycerol uptake. Northern blotting demonstrated that sbAQP was expressed at high levels in the posterior region of the gut, where two transcripts were identified (1.6·kb and 2·kb), and in kidney, where a single transcript was present (2·kb). In situ hybridisation studies with a sbAQP riboprobe revealed its presence in the lamina propria and smooth muscle layer of the posterior region of the gut and in epithelial cells of some kidney tubules. sbAQP was also present in putative chloride cells of the gill. Phylogenetic analysis of sbAQP, including putative GLP genes from Fugu rubripes, revealed that it did not group with any of the previously isolated vertebrate GLPs and instead formed a separate group, suggesting that it may be a novel GLP member.por
dc.description.sponsorshipThis work was supported by project PRAXIS XXI/2/2.1/BIA/211/94 from the Portuguese National Science and Technology Foundation (FCT), co-financed by EU structural funds, DG-Fisheries Project Q5RS-2002-00784 (CRYOCYTE) and an EU Biotech grant (QLRT2000-00778). C.R.A.S., J.C.R.C. and J.F. were in receipt of FCT fellowships PRAXIS XXI/BPD/22040/99, PRAXIS XXI/BD/19925/99BPD/22033/99, respectively.por
dc.identifier.citationC. R. A. Santos, M. D. Estêvão, J. Fuentes, J. C. R. Cardoso, M. Fabra, A. L. Passos, F. J. Detmers, P. M. T. Deen, J. Cerdà and D. M. Power, "Isolation of a novel aquaglyceroporin from a marine teleost (Sparus auratus) Function and tissue distribution" in The Journal of Experimental Biology 207, 1217-1227.por
dc.identifier.doihttp://dx.doi.org/10.1242/jeb.00867
dc.identifier.otherAUT: DPO00386; MES00382
dc.identifier.urihttp://hdl.handle.net/10400.1/5463
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherThe Company of Biologistspor
dc.subjectAquaporinpor
dc.subjectGLPpor
dc.subjectIn situ localisationpor
dc.subjectMultiple transcriptspor
dc.subjectFugu rubripespor
dc.subjectGastrointestinal tractpor
dc.subjectKidneypor
dc.subjectTeleost fishpor
dc.subjectSea breampor
dc.subjectSparus auratuspor
dc.titleIsolation of a novel aquaglyceroporin from a marine teleost (Sparus auratus) Function and tissue distributionpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1227por
oaire.citation.startPage1217por
oaire.citation.titleJournal of Experimental Biologypor
oaire.citation.volume207por
person.familyNameEstêvão
person.familyNameFuentes
person.familyNameCardoso
person.familyNamePower
person.givenNameMaria Dulce da Mota Antunes de Oliveira
person.givenNameJuan
person.givenNameJoão
person.givenNameDeborah Mary
person.identifierI-4695-2014
person.identifier392998
person.identifier14332
person.identifier.ciencia-id881B-53E8-0DB1
person.identifier.ciencia-id421B-E196-2C33
person.identifier.ciencia-id8B16-F203-2AFC
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-7151-8363
person.identifier.orcid0000-0003-0430-8734
person.identifier.orcid0000-0001-7890-0170
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-4151-2013
person.identifier.scopus-author-id8381590400
person.identifier.scopus-author-id7201832526
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id7101806760
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication67887883-2695-48cc-8221-aa46780ac143
relation.isAuthorOfPublicationd354b937-b18c-4ca5-a843-04a04269d60f
relation.isAuthorOfPublication1b670c84-15e3-4776-8871-50f9eb0eed0d
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery67887883-2695-48cc-8221-aa46780ac143

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Isolation of a novel aquaglyceroporin from a marine teleost (Sparus auratus) Function and tissue distribution.pdf
Size:
612.74 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.49 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)