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Production, release and olfactory detection of sex steroids by the tench (Tinca tinca L.)

2002Journal article Restricted access
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dc.contributor.authorPinillos, M. L.
dc.contributor.authorGuijarro, A. I.
dc.contributor.authorDelgado, M. J.
dc.contributor.authorHubbard, Peter
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorScott, A. P.
dc.date.accessioned2014-05-15T16:38:18Z
dc.date.available2014-05-15T16:38:18Z
dc.date.issued2002
dc.date.updated2014-05-13T10:24:41Z
dc.description.abstractThe present study is concerned with pheromone communication in tench (Tinca tinca L.), establishing firstly whethermales have a high olfactory sensitivity to some typical teleost sex steroids and prostaglandins; and secondly whether males and females might be able to synthesise and release some of these steroids into the water. The C21 steroid, 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P)was found to give large electro-olfactogram responses with an estimated threshold of detection of 10−12 M. The male tench were equally sensitive to glucuronidated 17,20β-P (10−11.6 M) but 100 times less sensitive to sulphated 17,20β-P (11−9.7 M). Preliminary data from cross-adaptation studies suggest that both the free and conjugated forms are detected by the same olfactory receptor(s). Male tench also had high olfactory sensitivity to prostaglandin F2α (PGF2α) and 15-keto PGF2α (11−11.5 and 10−11.4 M). They were relatively insensitive, however, to testosterone (T), androstenedione (AD), 11-ketotestosterone (11-KT), 17β-oestradiol (E2), 17,20β,21-trihydroxy-4-pregnen-3-one (17,20β,21-P) and 17,20α-dihydroxy-4-pregnen-3- one (17,20α-P). Radioimmunoassays were used to measure the steroids in plasma and water and all samples were processed for themeasurement of free, sulphated and glucuronidated fractions. In females, free 17,20β-P, 17,20α-P, free and glucuronidated T, and AD in plasma showed the largest increases in response to injection with mammalian gonadotropin-releasing hormone analogue (GnRHa) or Ovaprim (a mixture of GnRHa and a dopamine inhibitor). Free 17,20β-P was released into the water at the greatest rate. Plasma concentrations of the two conjugated forms of 17,20β-P were also elevated 18 h after the administration of GnRHa, but not by as much as the free steroid. In males, AD and 11-KT showed the greatest increase in response to GnRHa and were moreover released into the water at a higher rate in the treated group than in the control. The data support a possible pheromonal role for free and glucuronidated 17,20β-P.por
dc.identifier.citationPinillos, M. L.; Guijarro, A. I.; Delgado, M. J.; Hubbard, P. C.; Canario, A. V. M.; Scott, A. P.Production, release and olfactory detection of sex steroids by the tench (Tinca tinca L.), Fish Physiology and Biochemistry, 26, 2, 197-210, 2002.por
dc.identifier.doihttp://dx.doi.org/10.1023/A:1025421920443
dc.identifier.issn0920-1742
dc.identifier.otherAUT: ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3979
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringer Verlag, Kluwer Academic Publisherspor
dc.relation.publisherversionhttp://link.springer.com/article/10.1023%2FA%3A1025421920443por
dc.subjectElectro-olfactogrampor
dc.subjectPGF2αpor
dc.subject17,20β-Ppor
dc.subject17,20β-P-Glucuronidepor
dc.subject17,20β-P-sulphatepor
dc.subjectPheromonepor
dc.subjectReproductionpor
dc.subjectSteroidpor
dc.subjectTeleostpor
dc.subjectTenchpor
dc.titleProduction, release and olfactory detection of sex steroids by the tench (Tinca tinca L.)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage210por
oaire.citation.issue2
oaire.citation.startPage197por
oaire.citation.titleFish Physiology and Biochemistrypor
oaire.citation.volume26por
person.familyNameHubbard
person.familyNameCanario
person.givenNamePeter
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id951F-BA4B-A21F
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0002-3007-4647
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridB-2823-2008
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7005928467
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationf599b3ac-806b-4f65-9eb6-f70e68bcb142
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

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