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A glyphosate-based herbicide induces histomorphological and protein expression changes in the liver of the female guppy Poecilia reticulata

2017-02Journal article Restricted access
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dc.contributor.authorRezende dos Santos, Ana Paula
dc.contributor.authorLopes Rocha, Thiago
dc.contributor.authorBorges, Clayton Luiz
dc.contributor.authorBailao, Alexandre Melo
dc.contributor.authorde Almeida Soares, Celia Maria
dc.contributor.authorTeixeira de Saboia-Morais, Simone Maria
dc.date.accessioned2019-11-20T15:07:49Z
dc.date.available2019-11-20T15:07:49Z
dc.date.issued2017-02
dc.description.abstractGlyphosate-based herbicides (GBH) are among the most common herbicides found in aquatic systems, but limited data are available about their mode of action and hepatotoxicity in fish. This study investigated the hepatotoxicity induced by GBH in the guppy Poecilia reticulae using a histopathological assessment associated with a proteomic approach. Guppies were exposed to GBH for 24 h at 1.8 mg of glyphosate L-1, corresponding to 50% of the LC50, 96 h. The results indicate that the GBH at 1.8 mg of glyphosate L-1 induce the development of hepatic damage in P. reticulate, which is exposure-time dependent. The histopathological indexes demonstrate that GBH cause inflammatory, regressive, vascular and progressive disorders in the liver of guppies. Using 2D gel electrophoresis associated with mass spectrometry, 18 proteins that changed by GBH were identified and were related to the cellular structure, motility and transport, energy metabolism and apoptosis. The results show that the acute exposure to GBH causes hepatic histopathological damage related to protein expression profile changes in P. reticulate, indicating that a histopathological assessment associated with a proteomic analysis provides a valuable approach to assess the toxic effects of GBH in sentinel fish species. (C) 2016 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [038-PROCAD NF]
dc.description.sponsorshipNational Council for Scientific and Technological Development (CNPq) [473572/2010-9]
dc.identifier.doi10.1016/j.chemosphere.2016.10.116
dc.identifier.issn0045-6535
dc.identifier.issn1879-1298
dc.identifier.urihttp://hdl.handle.net/10400.1/13226
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPergamon-Elsevier Science Ltd
dc.subjectFish Prochilodus-Lineatus
dc.subjectTilapia Oreochromis-Niloticus
dc.subjectFresh-water fish
dc.subjectHepatocyte couplets
dc.subjectActive ingredient
dc.subjectDna-damage
dc.subjectRoundup(R)
dc.subjectExposure
dc.subjectCell
dc.subjectAngiogenesis
dc.titleA glyphosate-based herbicide induces histomorphological and protein expression changes in the liver of the female guppy Poecilia reticulata
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage943
oaire.citation.startPage933
oaire.citation.titleChemosphere
oaire.citation.volume168
person.familyNameLopes Rocha
person.givenNameThiago
person.identifier.orcid0000-0003-0551-6842
person.identifier.ridA-3864-2015
person.identifier.scopus-author-id37007842200
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublicationce186883-7988-4912-a5b7-39ce48201c27
relation.isAuthorOfPublication.latestForDiscoveryce186883-7988-4912-a5b7-39ce48201c27

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