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A new method for the study of essential fatty acid requirements in fish larvae

2009-05Journal article Open access
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dc.contributor.authorMorais, Sofia
dc.contributor.authorConceicao, Luis
dc.date.accessioned2018-12-07T14:57:52Z
dc.date.available2018-12-07T14:57:52Z
dc.date.issued2009-05
dc.description.abstractThis study describes a methodology with potential application in the estimation of essential fatty acid (EFA) requirements of fish larvae. Senegalese sole (Solea senegalensis) larvae were fed, from 16 days after hatching (DAH), on Artemia enriched with different oils, inducing graded dietary concentrations of DHA: (1) soyabean oil, containing no measurable amounts of DHA (NDHA); (2) fish oil, inducing a medium DHA level (MDHA, 3 g DHA/100 g fatty acids); and (3) a mixture of Easy DHA Selco and Microfeed, resulting in high DHA content (HDHA, 8 g/100 g). At 28 DAH a metabolic trial was conducted where larvae were tube fed [1-(14) C]DHA, in order to determine its absorption, retention in the gut and body tissues, as well as its oxidation. At 23 DAH the HDHA treatment induced a significantly higher larval growth, while at 32 DAH significant differences were only found between the NDHA and HDHA treatments. The absorption of tube-fed [1-(14) C]DHA was extremely high (94-95%) and independent of feeding regime. However, in larvae fed NDHA Artemia, a significantly higher amount of label was retained in the gut compartment and a concurrently lower retention was measured in the body. A significantly higher proportion of the absorbed DHA label was oxidized in larvae fed HDHA, compared to NDHA. Based on these results, we suggest that increasing dietary supply of DHA above the larval requirement level results in its increased oxidation for energy purposes and we propose potential applications of the tube feeding methodology using radiolabelled EFA in conjunction with dose-response studies.
dc.description.sponsorship'Fundacao para a Ciencia e a Tecnologia', Portugal [SFRH/BD/4902/2001]; FEDER, European Commission [PROMAR/SP5.P117/03]
dc.description.sponsorshipPROMAR/SP5.P117/03
dc.identifier.doi10.1017/S0007114508111436
dc.identifier.issn0007-1145
dc.identifier.urihttp://hdl.handle.net/10400.1/11742
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCambridge Univ Press
dc.relationDIGESTIVE AND ASSIMILATION CAPACITY OF MARINE FISH LARVAE WITH RESPECT TO DIETARY LIPID/PROTEIN RATIOS AND LIPID QUALITY
dc.subjectSolea-Senegalensis Larvae
dc.subjectArtemia Feeding Period
dc.subjectNeural Tissues
dc.subjectMarine Fish
dc.subjectGrowth
dc.subjectAbsorption
dc.subjectSurvival
dc.subjectTrout
dc.subjectNutrition
dc.subjectProfile
dc.titleA new method for the study of essential fatty acid requirements in fish larvae
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDIGESTIVE AND ASSIMILATION CAPACITY OF MARINE FISH LARVAE WITH RESPECT TO DIETARY LIPID/PROTEIN RATIOS AND LIPID QUALITY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F4902%2F2001/PT
oaire.citation.endPage1568
oaire.citation.issue10
oaire.citation.startPage1564
oaire.citation.titleBritish Journal of Nutrition
oaire.citation.volume101
person.familyNameConceicao
person.givenNameLuis
person.identifier1577554
person.identifier.ciencia-id9219-5DB3-CD39
person.identifier.orcid0000-0001-6102-2068
person.identifier.ridH-6257-2011
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication7e7768bc-83a8-4d03-9f62-ff0fa8f4d0d4
relation.isAuthorOfPublication.latestForDiscovery7e7768bc-83a8-4d03-9f62-ff0fa8f4d0d4
relation.isProjectOfPublication13b71c9d-c5a2-4df5-9bac-691e669c23f4
relation.isProjectOfPublication.latestForDiscovery13b71c9d-c5a2-4df5-9bac-691e669c23f4

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