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Fractioning macrocomponents of nannochloropsis oceanica by high-pressure homogenization, membrane processing, and ethanolic extraction

2026-01-25Artigo científico Acesso aberto
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datacite.subject.sdg12:Produção e Consumo Sustentáveis
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg02:Erradicar a Fome
dc.contributor.authorCunha, Pedro
dc.contributor.authorCarvalho, Bernardo
dc.contributor.authorKholany, Mariam
dc.contributor.authorCardoso, Helena
dc.contributor.authorPereira, Hugo
dc.contributor.authorVarela, João
dc.date.accessioned2026-07-09T13:20:02Z
dc.date.available2026-07-09T13:20:02Z
dc.date.issued2026-01-25
dc.description.abstractMulti-product biorefineries, which transform biomass feedstocks into multiple valuable bio-based products, are pivotal for transitioning from a fossil-based economy to a sustainable circular bioeconomy. This work proposes a processing pipeline for fractionating the macrocomponents of Nannochloropsis oceanica, which can serve as a basis for multi-product microalgae biorefineries. It consists of high-pressure homogenization (1200 bar, 1 cycle) to permeabilize the cells, and sequential membrane processing (0.2 µm dia-microfiltration followed by 100 kDa ultrafiltration) and ethanolic extraction (60 mL ethanol/g dry weight, 1 h) to fractionate the disrupted biomass. This biorefinery resulted in four final fractions: (1) enriched in water-soluble proteins (39.0 ± 2.8% w/w proteins; 10.7 ± 0.8% w/w carbohydrates); (2) remaining soluble components (5.7 ± 0.4% w/w proteins; 4.3 ± 0.9% w/w carbohydrates); (3) lipid-rich extract (62.4 ± 5.8% w/w lipids); and (4) non-extracted components (11.8 ± 4.5% w/w lipids), with mass recovery yields of 23.2 ± 2.1%, 6.9 ± 1.0%, 10.6 ± 1.9%, and 60.4 ± 4.1%, respectively. The ultrafiltration protein selectivity was not optimal, despite yielding a 2.6 times more concentrated fraction. Lipid extraction yield (35–60%) and purity (56–68%) were highly affected by the water content of the microfiltration retentate. Overall, 10.0 ± 0.9% of the proteins, 9.7 ± 1.8% of the carbohydrates, and 42.4 ± 13.4% of the lipids of N. oceanica were recovered in fractions 1, 2, and 3, respectively.eng
dc.identifier.doi10.3390/pr14030420
dc.identifier.issn2227-9717
dc.identifier.urihttp://hdl.handle.net/10400.1/29247
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofProcesses
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMicroalgae
dc.subjectMulti-product fractionation
dc.subjectMild processing
dc.subjectProcess integration
dc.subjectHigh-pressure homogenization
dc.subjectMembrane filtration
dc.subjectEthanolic extraction
dc.titleFractioning macrocomponents of nannochloropsis oceanica by high-pressure homogenization, membrane processing, and ethanolic extractioneng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/04326/2020
oaire.awardNumberLA/P/0101/2020
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.endPage17
oaire.citation.issue3
oaire.citation.startPage1
oaire.citation.titleProcesses
oaire.citation.volume14
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCarvalho
person.familyNameKholany
person.familyNamePereira
person.familyNameVarela
person.givenNameBernardo
person.givenNameMariam
person.givenNameHugo
person.givenNameJoão
person.identifier282781
person.identifier.ciencia-id331E-84FD-2F34
person.identifier.orcid0000-0003-4612-3797
person.identifier.orcid0000-0002-6279-2053
person.identifier.orcid0000-0002-1369-2099
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridE-9360-2015
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id54080259900
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication0655cee0-5f6a-407a-8ba6-bb446a45a9f4
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