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Improvement of the cryopreservation protocols for the dusky grouper, Epinephelus marginatus

2017-03Journal article Restricted access
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dc.contributor.authorF Riesco, Marta
dc.contributor.authorRaposo de Magalhães, Cláudia
dc.contributor.authorEngrola, Sofia
dc.contributor.authorMartinez-Paramo, Sonia
dc.contributor.authorMira, Sara
dc.contributor.authorCunha, Maria Emilia
dc.contributor.authorCabrita, Elsa
dc.date.accessioned2019-11-20T15:07:47Z
dc.date.available2019-11-20T15:07:47Z
dc.date.issued2017-03
dc.description.abstractThe dusky grouper, Epinephelus marginatus, is a potential species for aquaculture production although the limited number of males kept in captivity has been the cause of some constraints in its production. Sperm cryopreservation emerged as a solution for this problem. However, spermatozoa can be severely affected by freezing/thawing processes and poor sperm quality is a limiting factor in reproduction success. The present study aimed at evaluating two main aspects in the design of a cryopreservation protocol-extender additives (taurine, glucose, cholesterol, BSA) and sperm containers (0.5 mL straws, 2 mL cryovials and 5 mL macrotubes). Sperm quality was assessed through the evaluation of the percentage of motile cells, viable cells, DNA fragmentation, lipid peroxidation and apoptosis. Some specific techniques, such as Caspase 3/7 detection, which provides information on membrane integrity and cell death, detecting early and late apoptotic and necrotic events, were required to establish an optimized cryopreservation protocol for this species. Taurine was the most suitable cryopreservation additive in terms of viable cells and cholesterol presented the highest percentage of necrotic cells in this study. Caspase 3/7 assay enabled us to detect necrotic damage induced by cryopreservation. Statement of relevance: The development of reproductive tools in dusky grouper, a potential species for aquaculture production, emerges as important tool to decrease the number of wild males maintained in captivity. A cryopreservation protocol was previously described for this species although several constraints in terms of sperm quality were detected. Our work provided new evidences that cryopreservation protocols can be improved through the addition of certain additives and use of appropriate sperm containers. Specific sperm analysis was crucial to identify and establish the most suitable conditions for breeders management and species conservation purposes. (C) 2016 Elsevier B.V. All rights reserved.
dc.description.sponsorshipEuropean Social Fund [IF/00482/2014/CP1217/CT0005]
dc.description.sponsorshipOperational Programme Human Potential
dc.description.sponsorshipFoundation for Science and Technology of Portugal (FCT)
dc.description.sponsorshipPOCTEP programme under the European territorial cooperation objective [0251_ECOAQUA_5_E]
dc.description.sponsorshipEuropean Regional Development Fund (ERDF) Portugal Spain
dc.description.sponsorship[CCMAR/SC/BPD/09/2016]
dc.identifier.doi10.1016/j.aquaculture.2016.12,027
dc.identifier.issn0044-8486
dc.identifier.issn1873-5622
dc.identifier.urihttp://hdl.handle.net/10400.1/13211
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier Science Bv
dc.subjectRed-spotted grouper
dc.subjectSperm cryopreservation
dc.subjectOxidative stress
dc.subjectTaurine
dc.subjectSemen
dc.subjectAntioxidant
dc.subjectHypotaurine
dc.subjectSpermatozoa
dc.subjectParameters
dc.subjectExtender
dc.titleImprovement of the cryopreservation protocols for the dusky grouper, Epinephelus marginatus
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage213
oaire.citation.startPage207
oaire.citation.titleAquaculture
oaire.citation.volume470
person.familyNameF RIESCO
person.familyNameRaposo de Magalhães
person.familyNameEngrola
person.familyNameMartinez-Paramo
person.familyNameMira
person.familyNameCabrita
person.givenNameMARTA
person.givenNameCláudia
person.givenNameSofia
person.givenNameSonia
person.givenNameSara
person.givenNameElsa
person.identifier237846
person.identifier.ciencia-idEB1C-0E40-F003
person.identifier.ciencia-idA610-29E7-9B83
person.identifier.orcid0000-0002-3655-6401
person.identifier.orcid0000-0001-7751-2870
person.identifier.orcid0000-0002-5244-5541
person.identifier.orcid0000-0003-2899-0547
person.identifier.orcid0000-0001-8346-4449
person.identifier.orcid0000-0003-3864-8841
person.identifier.ridO-9766-2014
person.identifier.ridA-2485-2012
person.identifier.ridI-2700-2013
person.identifier.ridE-8523-2012
person.identifier.scopus-author-id54796976600
person.identifier.scopus-author-id8669620600
person.identifier.scopus-author-id16480798200
person.identifier.scopus-author-id6603417249
person.identifier.scopus-author-id26645697000
rcaap.rightsrestrictedAccess
rcaap.typearticle
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