Publication
CRISPR-based functional genomics in pluripotent stem cells
| dc.contributor.author | Zahedi, Setareh | |
| dc.date.accessioned | 2025-12-09T14:51:49Z | |
| dc.date.available | 2025-12-09T14:51:49Z | |
| dc.date.issued | 2025-11-14 | |
| dc.description.abstract | The integration of CRISPR-based functional genomics with pluripotent stem cell (PSC) technologies has been recognized as a transformative approach for investigating gene function, modeling human disease, and advancing regenerative medicine. The aim of this review is to provide a comprehensive evaluation of recent developments in CRISPR-Cas platforms, including gene knockouts, base and prime editing, and CRISPR activation or interference (CRISPRa/i), as applied to PSC systems. Studies employing human PSCs, including embryonic stem cells and induced pluripotent stem cells, have been examined to summarize methodologies for genome-wide screening, lineage tracing, and therapeutic engineering. Advances in editing efficiency, delivery strategies, and genomic safety have been reported, while limitations persist in the form of off-target modifications, epigenetic variability, and cell-type-specific responses. Notable applications include the generation of immune-evasive PSC lines, the development of organoid models for physiological and pathological studies, and the implementation of phenotypic screening for disease-relevant traits. Collectively, these technological and methodological advancements have established functional genomics of PSC-CRISPRSPR as a versatile and powerful framework for elucidating fundamental aspects of human biology, dissecting complex traits, and accelerating the translation of discoveries from experimental research to clinical implementation. | eng |
| dc.identifier.doi | 10.1007/s12015-025-11019-y | |
| dc.identifier.eissn | 2629-3277 | |
| dc.identifier.issn | 2629-3269 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/27935 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Springer | |
| dc.relation.ispartof | Stem Cell Reviews and Reports | |
| dc.rights.uri | N/A | |
| dc.subject | CRISPR | |
| dc.subject | CRISPR-Cas systems | |
| dc.subject | Pluripotent stem cells | |
| dc.subject | Embryonic stem cells | |
| dc.subject | Induced pluripotent stem cells | |
| dc.subject | Functional genomics | |
| dc.subject | Genome editing | |
| dc.subject | Organoids | |
| dc.subject | Regenerative medicine | |
| dc.title | CRISPR-based functional genomics in pluripotent stem cells | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.title | Stem Cell Reviews and Reports | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 |