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Fertilization capacity with rainbow trout DNA-damaged sperm and embryo developmental success

2010-06Journal article Open access
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dc.contributor.authorPerez-Cerezales, S.
dc.contributor.authorMartinez-Paramo, S.
dc.contributor.authorBeirao, J.
dc.contributor.authorHerraez, M. P.
dc.date.accessioned2018-12-07T14:53:33Z
dc.date.available2018-12-07T14:53:33Z
dc.date.issued2010-06
dc.description.abstractMammalian spermatozoa undergo a strong selection process along the female tract to guarantee fertilization by good quality cells, but risks of fertilization with DNA-damaged spermatozoa have been reported. In contrast, most external fertilizers such as fish seem to have weaker selection procedures. This fact, together with their high prolificacy and external embryo development, indicates that fish could be useful for the study of the effects of sperm DNA damage on embryo development. We cryopreserved sperm from rainbow trout using egg yolk and low-density lipoprotein as additives to promote different rates of DNA damage. DNA fragmentation and oxidization were analyzed using comet assay with and without digestion with restriction enzymes, and fertilization trials were performed. Some embryo batches were treated with 3-aminobenzamide (3AB) to inhibit DNA repair by the poly (ADP-ribose) polymerase, which is an enzyme of the base excision repair pathway. Results showed that all the spermatozoa cryopreserved with egg yolk carried more than 10% fragmented DNA, maintaining fertilization rates of 61.1+/-2.3 but a high rate of abortions, especially during gastrulation, and only 14.5+/-4.4 hatching success. Furthermore, after 3AB treatment, hatching dropped to 3.2+/-2.2, showing that at least 10% DNA fragmentation was repaired. We conclude that trout sperm maintains its ability to fertilize in spite of having DNA damage, but that embryo survival is affected. Damage is partially repaired by the oocyte during the first cleavage. Important advantages of using rainbow trout for the study of processes related to DNA damage and repair during development have been reported. Reproduction (2010) 139 989-997
dc.description.sponsorshipJunta de Castilla y Leon (Spain) [LE007A06]; University of Leon
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1530/REP-10-0037
dc.identifier.issn1470-1626
dc.identifier.urihttp://hdl.handle.net/10400.1/11569
dc.language.isoeng
dc.peerreviewedyes
dc.publisherBioscientifica Ltd
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectOncorhynchus-Mykiss
dc.subjectCryopreserved sperm
dc.subjectChromatin-structure
dc.subjectFish spermatozoa
dc.subjectIndividual cells
dc.subjectMale-infertility
dc.subjectSemen quality
dc.subjectFragmentation
dc.subjectRepair
dc.subjectCompetition
dc.titleFertilization capacity with rainbow trout DNA-damaged sperm and embryo developmental success
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage997
oaire.citation.issue6
oaire.citation.startPage989
oaire.citation.titleReproduction
oaire.citation.volume139
person.familyNameMartinez-Paramo
person.familyNameBeirão
person.familyNameHerraez
person.givenNameSonia
person.givenNameJosé
person.givenNamePaz
person.identifier.orcid0000-0003-2899-0547
person.identifier.orcid0000-0002-4444-7563
person.identifier.orcid0000-0002-0028-3616
person.identifier.ridI-2700-2013
person.identifier.ridK-6522-2012
person.identifier.ridC-6264-2013
person.identifier.scopus-author-id16480798200
person.identifier.scopus-author-id24480665300
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublicationed71a583-d3db-48b1-9675-ff023fda2e76
relation.isAuthorOfPublicationa4bcffd0-d38a-4d00-881e-2374d375f713
relation.isAuthorOfPublication51e0aac9-520e-46bc-b4c2-00fbd6cd39d4
relation.isAuthorOfPublication.latestForDiscovery51e0aac9-520e-46bc-b4c2-00fbd6cd39d4

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