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Development of tools to rapidly identify cryptic species and characterize their genetic diversity in different European kelp species

2021-10Journal article Open access
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dc.contributor.authorMauger, Stéphane
dc.contributor.authorFouqueau, Louise
dc.contributor.authorAvia, Komlan
dc.contributor.authorReynes, Lauric
dc.contributor.authorSerrao, Ester
dc.contributor.authorNeiva, J.
dc.contributor.authorValero, Myriam
dc.date.accessioned2021-11-02T10:19:33Z
dc.date.available2021-11-02T10:19:33Z
dc.date.issued2021-10
dc.description.abstractMarine ecosystems formed by kelp forests are severely threatened by global change and local coastline disturbances in many regions. In order to take appropriate conservation, mitigation, and restoration actions, it is crucial to identify the most diverse populations which could serve as a “reservoir” of genetic diversity. This requires the development of specifc tools, such as microsatellite markers to investigate the level and spatial distribution of genetic diversity. Here, we tested new polymorphic microsatellite loci from the genome of the kelp, Laminaria digitata, and tested them for cross-amplifcation and polymorphism in four closely related congeneric species (Laminaria hyperborea, Laminaria ochroleuca, Laminaria rodriguezii, and Laminaria pallida). Adding these 20 new microsatellite loci to the ten L. digitata loci previously developed by Billot et al. (Mol Ecol 7:1778-1780, 1998) and Brenan et al. (J R Soc Interface 11:1-12, 2014) and to the ten L. ochroleuca loci previ ously developed by Coelho et al. (Conserv Genet Resource 6:949-950, 2014), we retained a total of 30 polymorphic loci for L. digitata, 21 for L. hyperborea, 16 for L ochroleuca, 18 for L. rodriguezii, and 12 for L. pallida. These markers have been tested for the frst time in the last two species. As predicted, the proportion of markers that cross-amplifed between species decreased with increasing genetic distance. In addition, as problems of species identifcation were reported in this family, mainly between L. digitata and Hedophyllum nigripes, but also between L. digitata, L. hyperborea, and L. ochroleuca in areas where their range distributions overlap, we report a rapid PCR identifcation method based on species-specifc COI mitochondrial primers that allows these four species of kelp to be rapidly distinguished.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s10811-021-02613-xpt_PT
dc.identifier.eissn1573-5176
dc.identifier.urihttp://hdl.handle.net/10400.1/17278
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationNot Available
dc.relationHotspots de biodiversidade genética em florestas marinhas
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectGenetic diversitypt_PT
dc.subjectMicrosatellite markerspt_PT
dc.subjectCross-amplifcationpt_PT
dc.subjectSpecies identifcationpt_PT
dc.subjectBrown seaweedpt_PT
dc.titleDevelopment of tools to rapidly identify cryptic species and characterize their genetic diversity in different European kelp speciespt_PT
dc.title.alternativeDesenvolvimento de ferramentas para identificar rapidamente espécies enigmáticas e caracterizar sua diversidade genética em diferentes espécies Europeias de algaspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleNot Available
oaire.awardTitleHotspots de biodiversidade genética em florestas marinhas
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1361%2FCT0010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBSAB%2F150485%2F2019/PT
oaire.citation.titleJournal of Applied Phycologypt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamDL 57/2016
oaire.fundingStreamOE
person.familyNameSerrao
person.familyNameMiranda Neiva
person.givenNameEster A.
person.givenNameJoão
person.identifierC-6686-2012
person.identifierM-3818-2013
person.identifier.ciencia-id5B13-B26E-B1EC
person.identifier.ciencia-id191B-5464-41F5
person.identifier.orcid0000-0003-1316-658X
person.identifier.orcid0000-0002-5927-4570
person.identifier.scopus-author-id7004093604
person.identifier.scopus-author-id54895771800
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication45ccfe90-155c-4d6f-9e86-8f0fd064005f
relation.isAuthorOfPublication9e246701-725f-473c-afbd-6ec7cd92d759
relation.isAuthorOfPublication.latestForDiscovery9e246701-725f-473c-afbd-6ec7cd92d759
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