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Phylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis

2017Journal article Open access
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dc.contributor.authorFirmino, Joana
dc.contributor.authorCarballo, Carlos
dc.contributor.authorArmesto, Paula
dc.contributor.authorCampinho, Marco António
dc.contributor.authorPower, Deborah M.
dc.contributor.authorManchado, Manuel
dc.date.accessioned2018-12-07T14:53:38Z
dc.date.available2018-12-07T14:53:38Z
dc.date.issued2017
dc.description.abstractBackground: The identification of DNA methyltransferases (Dnmt) expression patterns during development and their regulation is important to understand the epigenetic mechanisms that modulate larval plasticity in marine fish. In this study, dnmt1 and dnmt3 paralogs were identified in the flatfish Solea senegalensis and expression patterns in early developmental stages and juveniles were determined. Additionally, the regulation of Dnmt transcription by a specific inhibitor (5-aza-2 '-deoxycytidine) and temperature was evaluated. Results: Five paralog genes of dnmt3, namely dnmt3aa, dnmt3ab, dnmt3ba, dnmt3bb. 1 and dnmt3bb. 2 and one gene for dnmt1 were identified. Phylogenetic analysis revealed that the dnmt gene family was highly conserved in teleosts and three fish-specific genes, dnmt3aa, dnmt3ba and dnmt3bb. 2 have evolved. The spatio-temporal expression patterns of four dnmts (dnmt1, dnmt3aa, dnmt3ab and dnmt3bb. 1) were different in early larval stages although all of them reduced expression with the age and were detected in neural organs and dnmt3aa appeared specific to somites. In juveniles, the four dnmt genes were expressed in brain and hematopoietic tissues such as kidney, spleen and gills. Treatment of sole embryos with 5-aza-2 '-deoxycytidine down-regulated dntm1 and up-regulated dntm3aa. Moreover, in lecithotrophic larval stages, dnmt3aa and dnmt3ab were temperature sensitive and their expression was higher in larvae incubated at 16 degrees C relative to 20 degrees C. Conclusion: Five dnmt3 and one dnmt1 paralog were identified in sole and their distinct developmental and tissue-specific expression patterns indicate that they may have different roles during development. The inhibitor 5-aza-2 '-deoxycytidine modified the transcript abundance of dntm1 and dntm3aa in embryos, which suggests that a regulatory feedback mechanism exists for these genes. The impact of thermal regime on expression levels of dnmt3aa and dnmt3ab in lecithotrophic larval stages suggests that these paralogs might be involved in thermal programing.
dc.description.sponsorshipINIA and EU through FEDER [RTA2013-00023-C0201]
dc.identifier.doi10.1186/s12861-017-0154-0
dc.identifier.issn1471-213X
dc.identifier.urihttp://hdl.handle.net/10400.1/11609
dc.language.isoeng
dc.peerreviewedyes
dc.publisherBiomed Central Ltd
dc.relationDevelopment of Microalgae-based novel high added-value products for the Cosmetic and Aquaculture industry
dc.relationFunctional biology of thyroid hormones in teleost development
dc.subjectMuscle-Fiber Recruitment
dc.subjectSalmon Salmo-Salar
dc.subjectGene-Expression
dc.subjectTeleost Fish
dc.subjectEmbryonic-Development
dc.subjectMolecular Characterization
dc.subjectEpigenetic Regulation
dc.subjectDicentrarchus-Labrax
dc.subjectThermal Plasticity
dc.subjectSomatic Growth
dc.titlePhylogeny, expression patterns and regulation of DNA Methyltransferases in early development of the flatfish, Solea senegalensis
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment of Microalgae-based novel high added-value products for the Cosmetic and Aquaculture industry
oaire.awardTitleFunctional biology of thyroid hormones in teleost development
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/691102/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F01274%2F2014%2FCP1217%2FCT0007/PT
oaire.citation.startPage11
oaire.citation.titleBmc Developmental Biology
oaire.citation.volume17
oaire.fundingStreamH2020
oaire.fundingStreamInvestigador FCT
person.familyNameCampinho
person.familyNamePower
person.givenNameMarco António
person.givenNameDeborah Mary
person.identifier.ciencia-id0E18-2560-6EC1
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-5238-0506
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridD-8833-2013
person.identifier.scopus-author-id8938999600
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryf8e8c466-6a6f-4ccf-b96d-34565fe53b6c
relation.isProjectOfPublication2488473c-6599-4873-9885-7638817cc0d5
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