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Teleost fish larvae adapt to dietary arachidonic acid supply through modulation of the expression of lipid metabolism and stress response genes

2012-09Journal article Open access
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dc.contributor.authorAlves Martins, Dulce
dc.contributor.authorRocha, Filipa
dc.contributor.authorMartinez-Rodriguez, Gonzalo
dc.contributor.authorBell, Gordon
dc.contributor.authorMorais, Sofia
dc.contributor.authorCastanheira, Maria Filipa
dc.contributor.authorBandarra, Narcisa
dc.contributor.authorCoutinho, Joana
dc.contributor.authorYufera, Manuel
dc.contributor.authorConceicao, Luis
dc.date.accessioned2018-12-07T14:58:16Z
dc.date.available2018-12-07T14:58:16Z
dc.date.issued2012-09
dc.description.abstractDietary fatty acid supply can affect stress response in fish during early development. Although knowledge on the mechanisms involved in fatty acid regulation of stress tolerance is scarce, it has often been hypothesised that eicosanoid profiles can influence cortisol production. Genomic cortisol actions are mediated by cytosolic receptors which may respond to cellular fatty acid signalling. An experiment was designed to test the effects of feeding gilthead sea-bream larvae with four microdiets, containing graded arachidonic acid (ARA) levels (0.4, 0.8, 1.5 and 3.0 %), on the expression of genes involved in stress response (steroidogenic acute regulatory protein, glucocorticoid receptor and phosphoenolpyruvate carboxykinase), lipid and, particularly, eicosanoid metabolism (hormone-sensitive lipase, PPAR alpha, phospholipase A(2), cyclo-oxygenase-2 and 5-lipoxygenase), as determined by real-time quantitative PCR. Fish fatty acid phenotypes reflected dietary fatty acid profiles. Growth performance, survival after acute stress and similar whole-body basal cortisol levels suggested that sea-bream larvae could tolerate a wide range of dietary ARA levels. Transcription of all genes analysed was significantly reduced at dietary ARA levels above 0.4%. Nonetheless, despite practical suppression of phospholipase A(2) transcription, higher leukotriene B-4 levels were detected in larvae fed 3.0% ARA, whereas a similar trend was observed regarding PGE(2) production. The present study demonstrates that adaptation to a wide range of dietary ARA levels in gilthead sea-bream larvae involves the modulation of the expression of genes related to eicosanoid synthesis, lipid metabolism and stress response. The roles of ARA, other polyunsaturates and eicosanoids as signals in this process are discussed.
dc.description.sponsorship'Fundacao para a Ciencia e a Tecnologia', (FCT, Portugal) [PTDC/MAR/67017/2006]; 'Consolider - Ingenio' programme (Plan Nacional I + D + I + FEDER, Spain [CSD2007-00002]; POCTEP Programme [0251-ECOAQUA-5-E]; FCT, Portugal [SFRH/BPD/32469/2006]; LARVANET [COST-STSM-FA0801-4496, COST-STSM-FA0801-5429]
dc.identifier.doi10.1017/S0007114511006143
dc.identifier.issn0007-1145
dc.identifier.urihttp://hdl.handle.net/10400.1/11941
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCambridge University Press
dc.relationTHE ROLE OF DIETARY LIPID ON THE STRESS RESPONSE BY SENEGAL SOLE SOLEA SENEGALENSIS KAUP LARVAE
dc.subjectAcute regulatory protein
dc.subjectSeabream Sparus-Aurata
dc.subjectPolyunsaturated-fatty-acids
dc.subjectProliferator-activated receptors
dc.subjectGilthead Seabream
dc.subjectGlucocorticoid-receptor
dc.subjectStimulated steroidogenesis
dc.subjectEicosapentaenoic acid
dc.subjectInterrenal cells
dc.subjectMessenger-Rnas
dc.titleTeleost fish larvae adapt to dietary arachidonic acid supply through modulation of the expression of lipid metabolism and stress response genes
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTHE ROLE OF DIETARY LIPID ON THE STRESS RESPONSE BY SENEGAL SOLE SOLEA SENEGALENSIS KAUP LARVAE
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F67017%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F32469%2F2006/PT
oaire.citation.endPage874
oaire.citation.issue5
oaire.citation.startPage864
oaire.citation.titleBritish Journal of Nutrition
oaire.citation.volume108
oaire.fundingStream3599-PPCDT
person.familyNameRocha
person.familyNameCastanheira
person.familyNameConceicao
person.givenNameFilipa
person.givenNameMaria Filipa
person.givenNameLuis
person.identifier1577554
person.identifier.ciencia-id9219-5DB3-CD39
person.identifier.orcid0000-0002-7531-9302
person.identifier.orcid0000-0001-5901-4077
person.identifier.orcid0000-0001-6102-2068
person.identifier.ridH-6257-2011
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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