Repository logo
 
Publication

Insights into core molecular changes associated with metamorphosis in gilthead seabream larvae across diverse hatcheries

2024-09Journal article Open access
Simple item page
dc.contributor.authorNAJAFPOUR, BABAK
dc.contributor.authorSantos, Soraia
dc.contributor.authorManchado, Manuel
dc.contributor.authorVidal, Aurora
dc.contributor.authorTsipourlianos, Andreas
dc.contributor.authorCanario, Adelino
dc.contributor.authorMoutou, Katerina A.
dc.contributor.authorPower, Deborah Mary
dc.date.accessioned2024-09-13T09:09:56Z
dc.date.available2024-09-13T09:09:56Z
dc.date.issued2024-09
dc.description.abstractEarly development is a critical period in fish aquaculture and is influenced by biotic and abiotic factors (e.g., temperature, feed) that can vary significantly between hatcheries, making it difficult to identify core factors determining quality. Many of the existing larval transcriptome studies are small-scale and occur under specific rearing conditions that do not mirror the diversity of larviculture practices at an industrial level. In the present transcriptome study, gilthead seabream at the larval to juvenile transition (metamorphosis) from several hatcheries in Europe (Greece, Italy, and France) were analysed in a large-scale RNA-seq study. The aim was to uncover the most significant molecular modifications occurring during metamorphosis, irrespective of differences in biotic or abiotic factors, to address knowledge gaps associated with critical early developmental stages under industrial hatchery conditions. Commonly modified gene transcripts between larval stages were identified based on the clustering of gene expression profiles of 25 gilthead seabream libraries from different hatcheries in a PCA analysis. When larvae at flexion were compared to larvae at mid-metamorphosis, 2243 differentially expressed genes (DEGs) were identified, and when larvae at early to mid-metamorphosis were compared to mid to late-metamorphosis, 2299 DEGs were identified. Comparative analysis across the developmental stages of gilthead seabream revealed genes of importance for the metamorphic transition and adaptation to rearing conditions, including genes related to the nervous system at flexion (24 days post hatch), enteroendocrine cell differentiation, and lipid homeostasis at early to mid-metamorphosis (46 dph), and enrichment of genes indicative of immune competence at mid to late-metamorphosis (51-54 dph). The differential expression of some endocrine-associated genes, dio1, dio2, cldn1, ing4, Pou3f4, and fgf22, highlights their importance in metamorphosis. Meta-analysis of the transcriptomes from two species, the gilthead seabream and Senegalese sole, that have differing symmetry and ecology uncovered common molecular expression patterns that underlie larvae maturation during metamorphosis, and we propose that these represent core gene markers of metamorphosis in these two fish species.eng
dc.identifier.doi10.1016/j.aquaculture.2024.740979
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.1/25880
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationConsumer driven Production: Integrating Innovative Approaches for Competitive and Sustainable Performance across the Mediterranean Aquaculture Value Chain
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofAquaculture
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCommercial hatcheries
dc.subjectCore genes of metamorphosis
dc.subjectDemersal
dc.subjectDevelopment
dc.subjectLarval transcriptome
dc.subjectPelagic
dc.titleInsights into core molecular changes associated with metamorphosis in gilthead seabream larvae across diverse hatcherieseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleConsumer driven Production: Integrating Innovative Approaches for Competitive and Sustainable Performance across the Mediterranean Aquaculture Value Chain
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/727610/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.startPage740979
oaire.citation.titleAquaculture
oaire.citation.volume590
oaire.fundingStreamH2020
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameNAJAFPOUR
person.familyNameSantos
person.familyNameCanario
person.familyNamePower
person.givenNameBABAK
person.givenNameSoraia
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifier143624
person.identifier.ciencia-idEF18-F0AF-D388
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-2977-482X
person.identifier.orcid0000-0001-5006-2021
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridC-1328-2018
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication8f1689bd-f300-49d5-9d7a-5ad8ccbe51af
relation.isAuthorOfPublicationcf930acd-5641-45b4-8a7c-5b6c492df42d
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery8f1689bd-f300-49d5-9d7a-5ad8ccbe51af
relation.isProjectOfPublication00a40603-7d09-4e16-86a2-a9ddd656ae46
relation.isProjectOfPublicationfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f
relation.isProjectOfPublication15f91d45-e070-47d8-b6b8-efd4de31d9a8
relation.isProjectOfPublication794d4c77-c731-471e-bc96-5a41dcd3d872
relation.isProjectOfPublication.latestForDiscovery00a40603-7d09-4e16-86a2-a9ddd656ae46

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
1-s2.0-S004484862400440X-main.pdf
Size:
3.22 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)